干涉鸡热休克蛋白70基因表达诱导鸡胚成纤维细胞凋亡

Abstract

Abstract:

The purpose of this paper is to study the relationship and molecular mechanisms between chicken heat shock protein 70 (HSP70) and apoptosis. Plasmid vectors expressing short hairpin-like structure small interfering RNA (shRNA) targeting chicken HSP70 were constructed and transfected into the chicken embryo fibroblast (CEF) in this study. At 18, 24, 28 h post interference, the CEF apoptosis was observed by Annexin V-FITC method following interference of hsp70 expression and caspase3 ＆ caspase8 were quantified by real-time RT-PCR. The results showed that, compared with the mock cell, 1) Apoptotic cells increased as the interference time extended. 2) The hsp70 in CEF cells decreased by 75%, 83% and 96% while caspase3 increased to 250%, 140% and 110%, and caspase8 decreased to 76%, 80% and 34% at 18, 24, 28 h post interference respectively. The results projected that chicken hsp70 protects the CEF cells against apoptosis mainly via the mitochondrial pathway.

CLC Number:

S852.23

YANG Fan, TANG Cheng, YUE Hua. Interference of Chicken Heat Shock Protein 70 Gene Expression Induced ChickenEmbryo Fibroblast Apoptosis[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, doi: .

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Interference of Chicken Heat Shock Protein 70 Gene Expression Induced ChickenEmbryo Fibroblast Apoptosis

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