孕酮、干扰素τ对牛子宫内膜上细胞外基质及其相关配体表达的影响

Abstract

Abstract:

To reveal the effects of interferon τ (IFN-τ) and progesterone on the expression level of extracellular matrix (ECM) and their ligands genes in bovine endometrial cells (bECs). In the study, bECs were cultured in medium containing bovine IFN-τ (bIFN-τ), progesterone and combination of bIFN-τ and progesterone, respectively, and then expression level of ECM related genes (Collagen, laminin, Fibronectin, THBS and Tenascin) and their ligand genes (Syndecan, CD44, CD47 and RHAMM) in bECs were detected by quantitative PCR after in vitro cultured bECs were treated for 24 h with bIFN-τ, progesterone, combination of bIFN-τ and progesterone, respectively. The results showed that, bIFN-τ significantly induce the expression of Tenascin, Collagen, RHAMM and Laminin, and progesterone significantly induced the expression of CD44, Fibronectin, RHAMM, and Laminin, but under co-action of bIFN-τ and progesterone, the expression level of CD44 and THBS was inhibited, and the expression of Tenascin, Fibonectin and Collagen returned to the expression level in the control group. The results suggest that bIFN-τ and progesterone might regulate the expression of ECM and their ligands in the process of embryo implantation.

CLC Number:

S823
S814.8

ZHANG Ming, ZHENG Jie, GAN Xiao, CHEN Shi-yi, ZENG Chang-jun, ZHOU Guang-bin, LAI Song-jia. Effects of Progesterone，Interferon-τ on Expression of the Related Genes of Extracellular Matrix-Their Ligands in Cultured Bovine Endometrial Cells[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, doi: .

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References

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Effects of Progesterone，Interferon-τ on Expression of the Related Genes of Extracellular Matrix-Their Ligands in Cultured Bovine Endometrial Cells

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