Abstract: To explore the sequence structure and the regulation mechanism of goat MyoG gene in muscle differentiation, the partial DNA sequences (2 363 bp) were obtained for MyoG gene of Boer goat by direct sequencing, and the sequences were analyzed in other species. The results showed that the sequences of Boer goat MyoG gene consisted of 3 exons, 2 introns and some of 5′ (74 bp) and 3′ (260 bp) untranslated region, with 675 bp coding sequences, encoding a total of 224 amino acids. Structural analysis indicated that the peptide chain had not signal peptide, and a bHLH domain was located at No. 1-138 amino acids. Compared Boer goat MyoG with other species, the results showed that the homology of nucleotide of coding region and amino acid accorded with genetic relationship among the species. The cluster result of un-rooted phylogenetic tree was similar to the physiological characteristics of the species and their traditional classification. Identification and sequence analysis of Boer goat MyoG gene provided the important biological information for researching the mechanism of goat′s muscle development and regulation, artificially improving the muscle quality of goat.