B亚型禽偏肺病毒病弱毒疫苗对商品蛋鸡的免疫效果

doi:10.11843/j.issn.0366-6964.2025.08.033

Abstract

Abstract:

To evaluate the protective efficacy of the attenuated vaccine against subtype B avian metapneumovirus on commercial layer chickens, the attenuated vaccine against subtype B avian metapneumovirus (LN16-A strain) prepared in the laboratory was used to immunize 21-day-old commercial layer chickens by intranasal administration at a dose of 400 TCID₅₀/chicken. Samples were collected on 7, 14, and 21 days post-immunization to separate the serum and measure the ELISA antibody and neutralizing antibody titers. At 21 days after immunization, the results showed the positive rate of ELISA antibody was 90% and the average neutralizing antibody titer was 7.51 log2 in the immunization group. At 21 days post-immunization, the commercial layer chickens were attacked by subtype B avian metapneumovirus (LN16-V strain) at a dose of 5 000 TCID₅₀ per chicken. The results indicated that chickens in the challenge control group had cloudy or viscous nose with an 80% incidence rate, while chickens in the immune group showed no clinical symptoms. RT-qPCR was used to measure virus copies in palatine swabs collected from chickens in each group from 1 to 7 days. The results showed that the viral loads in the immune group decreased by 175 folds compared with that in the challenge control group. And the histopathological examination results showed that varying degrees of pathological damage in tissues such as the turbinate bone and trachea of chickens were found in the challenge control group, while no obvious pathological changes of chickens were found in the immune group. The above results indicate that the attenuated vaccine against subtype B avian metapneumovirus (LN16-A strain) can provide protection for commercial layer chickens. And this study further provides a theoretical basis for controlling the prevalence of subtype B avian metapneumovirus in layer flocks and formulating an immunization program to prevent subtype B avian metapneumovirus disease.

Key words: subtype B avian metapneumovirus, attenuated vaccine, commercial layer chickens, immune effect

CLC Number:

S852.659.5

XUE Xiaoxiao, MENG Lingzhai, WANG Suyan, YU Mengmeng, CHEN Yuntong, QI Xiaole, LI Liuan, YU Xiaoxue, GAO Yulong. Study on Immune Effect of Subtype B Attenuated Avian Metapneumovirus Disease Vaccine on Commercial Layer Chickens[J]. Acta Veterinaria et Zootechnica Sinica, 2025, 56(8): 3958-3966.

Figures/Tables 8

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引物及探针 Primer and probe	引物及探针序列(5′→3′) Primer and probe sequence
aMPV-B-F	AATAGTCCTCAAGCAAGTCCTCAG
aMPV-B-R	CTGTTGTAATTTGACCTGTTCTACACT
aMPV-B-P	FAM-CTGGTGTTATCAGCCTTAGGCTTGACGCT-TAMRA

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Study on Immune Effect of Subtype B Attenuated Avian Metapneumovirus Disease Vaccine on Commercial Layer Chickens

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