Abstract:

Periodontitis is one of the common oral diseases in dogs, which can cause irreversible periodontal tissue loss and reduced periodontal adhesion, and seriously affect animal welfare. This study was designed to understand the drug resistance status oral flora in canine periodontitis; and to explore the drug sensitivity and drug resistance genes of characteristic pathogenic bacteria of canine periodontitis. This trial will be randomly selected periodontitis-infected dogs and healthy dogs to collect dental plaque and conduct drug sensitivity tests. In addition, 16S rDNA sequencing was performed on oral samples. The differences in oral flora between periodontitis-infected dogs and healthy dogs were explored, and the possible characteristic pathogenic bacteria causing canine periodontitis were identified. The drug susceptibility of the oral flora was determined. Then, the isolation, identification and drug sensitivity test were performed. The results showed that the relative abundance of Bacteroidetes and Spirochaeta increased at the level of oral flora, and the relative abundance of Porphyromonas at the genus level was significantly increased. It was speculated that the potential pathogenic bacteria of canine periodontitis was Porphyromonas. The subgingival aerobic bacteria had the highest sensitivity to amoxicillin and enrofloxacin, and had the lowest sensitivity to metronidazole. The sensitivity of subgingival anaerobes to enrofloxacin was medium, and the sensitivity to metronidazole was lowest. P. clinical is sensitive to enrofloxacin and contains two resistance genes ermF and tetQ. Enrofloxacin is preferred in the clinical treatment of canine periodontitis.

Key words: canine periodontitis, isolation and identification of flora, drug sensitivity test, drug resistance gene