Table 1

PCR primer sequence of truncated IL-1β protein"

Table 2

Clinical score of tent mice and evaluation criteria"

Fig. 1

SDS-PAGE and Western blot identification of recombinant IL-1β protein A. SDS-PAGE identification of recombinant protein expression; B. SDS-PAGE identification of inclusion body purification; C. Western blot detection of IL-1β protein; M. Protein marker; 1. The whole bacteria of recombinant protein without induction; 2. Whole bacteria expression with induction; 3. Lysate supernatant with induction; 4. Lysate precipitation with induction; 5. Empty vector transformed bacteria lysate with induction; 6. Purified recombinant IL-1β protein"

Fig. 2

Western blot characterization of the reactivity of monoclonal antibodies to IL-1β protein with eukaryotic systems expressing IL-1β protein M. Protein marker; 1. Transfection of HEK 293T cells with pCAGGS-IL-1β; 2. HEK 293T cells"

Fig. 3

Identification of recombinant IL-1β protein truncator expression by SDS-PAGE and Western blot A. Identification of truncated body protein by SDS-PAGE; B. Identification of truncated body protein by Western blot. M. Protein marker; 1-12. Truncated protein F1-F12; 13. pGEX-4T-1"

Fig. 4

Construction strategy of the IL-1β truncated protein and epitope mapping A. The truncated IL-1β protein and their reaction with mAb. B. The region recognized by the mAb"

Fig. 5

Amino acid sequence comparison of the epitopes of porcine IL-1β, recognized by the mAb, with those of mouse IL-1β Sus. Porcine; Mus. Mouse"

Fig. 6

The levels of NO, TNF-α, IL-6 and IL-1β in mouse serum detected with ELISA # is the difference between the LPS control group and the blank group, #. P<0.05, ##. P<0.01, ###. P<0.001, ####. P<0.000 1; * is the difference between the treatment group and LPS control group, *. P<0.05, **. P<0.01, ***. P<0.001, ****. P<0.000 1; ns. The difference is not significant"

Table 3

Clinical scoring statistics in mice ($\bar x \pm s$)"