北京黑猪HoxB簇基因多态性与脊椎数及胴体性状的关联分析

doi:10.11843/j.issn.0366-6964.2023.01.011

Abstract

Abstract: The aim of this study was to investigate the association between polymorphisms of 9 genes (HoxB1-7,9 and 13) in HoxB cluster and vertebral number and carcass traits (carcass length, straight length, diagonal length and carcass weight) in Beijing black pigs. In this study, DNA was extracted from ear tissue of 251 healthy Beijing black pigs at the age of 220 days. The SNPs of 9 HoxB genes were genotyped by primer design, polymerase chain reaction (PCR) and Sanger sequencing, and the genotype frequency and allele frequency distribution of the mutated loci were calculated. The association analysis was performed between their polymorphism and the vertebral number and carcass traits. A total of 12 non-coding region (UTR) SNPs were detected in 4 genes, including one 5'UTR mutation of HoxB2, two 3'UTR mutation of HoxB5, two 3'UTR mutation of HoxB9 and seven 3'UTR mutation of HoxB13. Statistical analysis using Duncan’s multiple test revealed that a 5'UTR mutation of HoxB2 (c.40 C>T) and two 3'UTR mutations of HoxB5(c.1766 A>G and c.1767 A>G) associated with these traits indistinctively (P>0.05). A 3'UTR mutation of HoxB9 (c.2743 C>T) were significantly associated with carcass traits(P<0.05), and two 3'UTR mutations of HoxB13 (c.1863 G>A, c.1440 A>C) significantly associated with the vertebral number (P<0.05). In conclusion, among the 9 HoxB cluster genes, only one 3'UTR mutation (c.2743 C>T) in HoxB9 gene was significantly associated with carcass traits (P<0.05), two 3'UTR mutations (c.1863 G>A, c.1440 A>C) of HoxB13 gene were significantly associated with the vertebral number (P<0.05). Above the 3 SNPs could be used as candidate functional loci for the variation of vertebral number and carcass traits of Beijing black pigs.

Key words: Beijing black pigs, HoxB9, HoxB13, vertebral number, carcass traits

CLC Number:

S828.2

NIU Naiqi, SU Yanfang, YANG Man, HOU Xinhua, WANG Ligang, ZHANG Longchao. Association Analysis of Polymorphisms of HoxB Cluster Genes with Vertebral Number and Carcass Traits in Beijing Black Pigs[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(1): 113-121.

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Association Analysis of Polymorphisms of HoxB Cluster Genes with Vertebral Number and Carcass Traits in Beijing Black Pigs

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