共轭亚油酸诱导的奶牛低脂乳症中脂肪球膜甘油磷脂的组成差异分析

doi:10.11843/j.issn.0366-6964.2022.03.012

Abstract

Abstract: The purpose of this study was to analyze the content of glycerolphospholipids in fat globule membrane in dairy cows with low-fat depression induced by conjugated linoleic acid (CLA). Ten Holstein cows with similar body conditions and at mid-lactation were selected for their own before and after control design. The test was carried out for 12 days, cows were fed with feeding basal diet +400 g CLA every day from day 1 to day 6, and then changed to the basal diet from day 7 to day 12. Cow performance, milk composition and milk fat globules(MFG) size parameters were measured daily. The milk samples were analyzed for glycerophospholipid on the first、7th and 12th day by mass spectrometry. The results showed that CLA treatment had no significant effect on the milk production, feed intake, milk protein and lactose content of dairy cows (P>0.05), but significantly reduced the milk fat rate of dairy cows (P<0.05). The size of fat globules average surface area diameter (D_[3,2])、10% particle volume diameter (Dv(10)) and 50% particle volume diameter (Dv(50)) all showed a trend of first decreased and then increased (P<0.05), while the specific surface area (SSA) first increased and then decreased (P<0.05). With the extension of feeding time, the small MFG distribution gradually increased and then decreased, while the large MFG first decreased and then gradually increased (P<0.05). Analysis of glycerophospholipids found that the concentration of phosphatidylcholine (PC 28:0, PC 30:0, PC 32:0, PC 34:1, PC 36:4 and PC 38:5) and phosphatidylethanolamine (PE 28:0, PE 30:0, PE 31:1e, PE 32:0, PE 32:2e, PE 33:2e, PE 34:0, PE 34:1, PE 34:2, PE 34:2e, PE 34:4e, PE 34:5e, PE 35:1, PE 35:3e, PE 35:5e, PE 36:2e, PE 36:4, PE 36:5e, PE 38:3, PE 38:4) showed a trend of first decreasing and then increasing. And content of phosphatidylinositol (PI) increased. These results indicate that CLA affect the size of fat globules and content and composition of phospholipids in milk fat globule membrane of dairy cows.

Key words: milk fat globules, conjugated linoleic acid, particle size, glycerophospholipids

CLC Number:

S823.91

YANG Jingna, HU Mingyue, LU Wenyan, HUANG Qixue, WEI Anqi, WANG Yueying, ZHONG Kai, HAN Liqiang. Analysis of Milk Fat Globule Membrane Glycerolphospholipids with Low-fat Depression Induced by Conjugated Linoleic Acid in Dairy Cows[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(3): 791-801.

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Analysis of Milk Fat Globule Membrane Glycerolphospholipids with Low-fat Depression Induced by Conjugated Linoleic Acid in Dairy Cows

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