不同生长时期梅花鹿鹿茸代谢组分析

doi:10.11843/j.issn.0366-6964.2022.12.037

Abstract

Abstract: The purpose of this study was to explore the differential expression of small molecule metabolites in sika deer antler in different growth stages, and to lay a foundation for the study of the molecular mechanism of rapid growth and ossification of deer antler. Four years old male Northeast Sika deer in good condition were selected, and antler growing for 25, 45, 65, 100 and 130 d were collected as experimental samples, with 3 biological replicates in each period. Metabolomic analysis of the samples was performed by UHPLC-TOF-MS. The results of principal component analysis and hierarchical cluster analysis showed that the 5 growth periods could be divided into two stages: growth stage (25, 45 and 65 d) and ossification stage (100 and 130 d). A total of 171 significantly differentially expressed metabolites (VIP>1, P<0.05, |fold change|>2) were screened and identified, which were compared to 112 metabolites in the HMDB database, divided into 7 superclass. The contents of L-pyroglutamic acid, L-histidine, L-carnosine and acemic acid were the highest at 100 days, and 15-deoxy-δ12, 14-prostaglandin J2, prostaglandin H2 and prostaglandin I2 were significantly up-regulated at 130 days, while the expressions of other amino acids and organic acids were all up-regulated during the growth phase. The differential metabolites were significantly enriched in 13 KEGG metabolic pathways, such as aminoacyl-trNA biosynthesis and histidine metabolism. This study results provides data support for exploring the molecular mechanism of rapid growth and ossification of antler at the metabolome level.

Key words: sika deer, antler, different growth stages, UHPLC-TOF-MS

CLC Number:

S825.2

ZHANG Ranran, RONG Min, DONG Yimeng, WANG Tianjiao, XING Xiumei. Metabolomic Analysis of Sika Deer Antler in Different Growth Stages[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(12): 4518-4526.

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Metabolomic Analysis of Sika Deer Antler in Different Growth Stages

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