N-氨甲酰谷氨酸对荷斯坦奶公牛生长性能、瘤胃发酵及微生物区系和甲烷排放量的影响

doi:10.11843/j.issn.0366-6964.2020.08.014

Abstract

Abstract: The present study was conducted to investigate the effects of dietary supplementation of N-carbamylglutamate (NCG) on growth performance, rumen fermentation, rumen microflora and methane emission of Holstein bulls. Forty-five healthy Holstein bulls (body weight (478±13.66) kg) were randomly assigned into 3 treatments with 15 replicates in each treatments, the bulls were supplemented with 0 (group Ⅰ, control), 15 (group Ⅱ) and 25 g·d^-1 (group Ⅲ) of NCG per head, respectively. A 7-day adaptation period and a 90-day experimental period were included in this study. The results showed that: 1) The feed-gain ratio (F/G) was reduced by 6.91% (P<0.01) and 7.91%(P<0.01) in bulls fed 15, 25 g·d^-1 NCG, respectively, compared with control; 2) The microbial protein concentration in rumen increased by 22.91% (P<0.01) and 15.17%(P<0.01) of bulls fed 15, 25 g·d^-1 NCG, respectively, compared with control; The concentrations of propionic acid in rumen increased linearly (P<0.01) while NCG supplementation increased, with the highest value for 15 g·d^-1 NCG group; 3) From the analysis of the relative abundance of rumen methanogenus at genus level, the relative abundances of Methanobrevibacter in rumen was the lowest when supplemented with 25 g·d^-1 NCG, which relative abundance was 80.05%;The relative abundance of Methanosphaera and Methanosaeta in rumen decreased linearly (P<0.01) while NCG supplementation increased; 4) Dietary supplemented with different levels of NCG all decreased the methane emission of Holstein bulls,the methane emission were decreased by 8.44% and 10.51% in bulls fed 15, 25 g·d^-1 NCG (P<0.01), respectively, compared with control. In conclusion, under the conditions of this experiment, supplemented wiht NCG can effectively regulate rumen fermentation of Holstein bulls, improve the efficiency of feed conversion ratio and reduce methane emissions. The recommended NCG supplementation level is 15 g·d^-1.

Key words: N-carbamylglutamate, Holstein bulls, feed-gain ratio, microflora, methane emission

CLC Number:

S823.9

SUN Xueli, LI Qiufeng, CAO Yufeng, YAN Jinling, LIU Taotao, ZHANG Yixian, LI Jianguo, GAO Yanxia, LI Shujing, XIE Peng, ZHANG Xiujiang. Effects of Dietary Addition of N-carbamylglutamate on Growth Performance, Rumen Fermentation， Rumen Microflora and Methane Emission of Holstein Bulls[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(8): 1903-1912.

References 44

[1]	ABDU S B,EHOCHE O W,ADAMU A M,et al.Ruminant livestock and greenhouse-gases (a Nutritionist PersPective)[J].IJAS,2012,2(3):223-225.
[2]	袁楷,卢建,朱寅彪,等.调控反刍家畜瘤胃甲烷生成的措施[J].畜禽业,2008(2):66-68.YUAN K,LU J,ZHU Y B,et al.The strategies of mitigating ruminal methane production[J].Livestock and Poultry Industry,2008(2):66-68.(in Chinese)
[3]	安娟,赵晓川.反刍动物甲烷排放机制及其调控[J].饲料工业,2006,27(13):57-59.AN J,ZHAO X C.Mechanism and methods of regulation for ruminent methane emissions[J].Feed Industry,2006,27(13):57-59.(in Chinese)
[4]	JOHNSON K A,JOHNSON D E.Methane emissions from cattle[J].J Anim Sci,1995,73(8):2483-2492.
[5]	苏醒,董国忠.反刍动物甲烷生成机制及调控[J].中国草食动物,2010,30(2):66-69.SU X,DONG G Z.Mechanism and regulation of methane production in ruminants[J].China Herbivores,2010,30(2):66-69.(in Chinese)
[6]	班志彬,杨华明,梁浩,等.食品级硝酸盐在反刍动物甲烷减排中的运用[J].吉林农业科学,2014,39(6):51-54.BAN Z B,YANG H M,LIANG H,et al.Use of dietary nitrates in reducing methane emissions of ruminant[J].Journal of Jilin Agricultural Sciences,2014,39(6):51-54.(in Chinese)
[7]	吕永艳,刘建雷,胡昌军,等.全棉籽对瘤胃内环境及纤维素酶活性的影响[J].饲料研究,2011(6):9-12.LV Y Y,LIU J L,HU C J.et al.Effect of cottonseed on rumen internal environment and cellulase activity[J].Feed Research,2011(6):9-12.(in Chinese)
[8]	花万里,张振伟,石绘陆.莫能菌素对反刍动物的作用机理及影响[J].养殖与饲料,2012(10):33-34.HUA W L,ZHANG Z W,SHI H L.Mechanism and effect of monensin on ruminants[J].Animals Breeding and Feed,2012(10):33-34.(in Chinese)
[9]	杨晓燕,赵补军,国卫杰.莫能菌素在牛生产中的应用[J].新疆畜牧业,2011(S1):4-5.YANG X Y,ZHAO B J,GUO W J.Application of monensin in animal production[J].Xinjiang Animal Husbandry,2011(S1):4-5.(in Chinese)
[10]	SANTOSO B,MWENYA B,SAR C,et al.Effects of supplementing galacto-oligosaccharides,Yucca schidigera or nisin on rumen methanogenesis,nitrogen and energy metabolism in sheep[J].Livest Prod Sci,2004,91(3):209-217.
[11]	LILA Z A,MOHAMMED N,KANDA S,et al.Effect of sarsaponin on ruminal fermentation with particular reference to methane production in vitro[J].J Dairy Sci,2003,86(10):3330-3336.
[12]	张华,童津津,孙铭维,等.植物提取物对反刍动物瘤胃发酵、生产性能及甲烷产量的调控作用及其机制[J].动物营养学报,2018,30(6):2027-2035.ZHANG H,TONG J J,SUN M W,et al.Regulation of plant extracts on rumen fermentation,performance and methane production in ruminants and its mechanisms[J].Chinese Journal of Animal Nutrition,2018,30(6):2027-2035.(in Chinese)
[13]	KIM S W,WU G Y.Regulatory role for amino acids in mammary gland growth and milk synthesis[J].Amino Acids,2009,37(1):89-95.
[14]	李新花,赖玉娇,王春平.N-氨甲酰谷氨酸在反刍动物生产中应用研究进展[J].中国奶牛,2016(2):8-11.LI X H,LAI Y J,WANG C P.The development and application of N-carbamylgluatmate in ruminant production[J].China Dairy Cattle,2016(2):8-11.(in Chinese)
[15]	刘南南.日粮碳水化合物平衡指数和延胡索酸对山羊瘤胃发酵、微生物区系和甲烷产生的影响[D].杨凌:西北农林科技大学,2014.LIU N N.Effects of dietary physical effective fiber to rumen degradable starch ratios and fumaric acids on rumen fermentation,microbial communities and methane emission in dairy goats[D].Yangling:Northwest A&F University,2014.(in Chinese)
[16]	冯志华.蒺藜皂苷对奶牛瘤胃发酵、甲烷产量、抗氧化功能及免疫功能的影响[D].保定:河北农业大学,2013.FENG Z H.Effects of gross saponin of tribulus terrestris on rumen fermentation,methane production,antioxidant capacity and immune function in dairy cows[D].Baoding:Hebei Agricultural University,2013.(in Chinese)
[17]	冯宗慈,高民.通过比色测定瘤胃液氨氮含量方法的改进[J].畜牧与饲料科学,2010,31(6-7):37.FENG Z C,GAO M.Improvement of the method for determining the content of ammonia nitrogen in rumen fluid by colorimetry[J].Animal Husbandry and Feed Science,2010,31(6-7):37.(in Chinese)
[18]	王加启.反刍动物营养学研究方法[M].北京:现代教育出版社,2011.WANG J Q.Research methods of ruminant nutrition[M].Beijing:Modern Education Press,2011.(in Chinese)
[19]	COTTA M A,RUSSELL J B.Effect of peptides and amino acids on efficiency of rumen bacterial protein synthesis in continuous culture[J].J Dairy Sci,1982,65(2):226-234.
[20]	HAAS B J,GEVERS D,EARL A M,et al.Chimeric 16S rRNA sequence formation and detection in Sanger and 454-pyrosequenced PCR amplicons[J].Genome Res,2011,21(3):494-504.
[21]	EDGAR R C.UPARSE:highly accurate OTU sequences from microbial amplicon reads[J].Nat Methods,2013,10(10):996-998.
[22]	WANG Q,GARRITY G M,TIEDJE J M,et al.Naive bayesian classifier for rapid assignment of rRNA sequences into the new bacterial taxonomy[J].Appl Environ Microbiol,2007,73(16):5261-5267.
[23]	DEMEYER D I.Rumen microbes and digestion of Plant cell walls[J].Agric Environ,1981,6(2-3):295-337.
[24]	COBELLIS G,YU Z T,FORTE C,et al.Dietary supplementation of Rosmarinus officinalis L. leaves in sheep affects the abundance of rumen methanogens and other microbial populations[J].J Anim Sci Biotechnol,2016,7:27.
[25]	KIM J,ERIKSON D W,BURGHARDT R C,et al.Secreted phosphoprotein 1 binds integrins to initiate multiple cell signaling pathways,including FRAP1/mTOR,to support attachment and force-generated migration of trophectoderm cells[J].Matrix Biol,2010,29(5):369-382.
[26]	MCKNIGHT J R,SATTERFIELD M C,JOBGEN W S,et al.Beneficial effects of L-arginine on reducing obesity:potential mechanisms and important implications for human health[J].Amino Acids,2010,39(2):349-357.
[27]	朱建津,乐国伟,施用晖,等.动物采食量生理调控物质的研究进展[J].饲料工业,2004,25(4):26-28.ZHU J J,LE G W,SHI Y H,et al.Headway of study the physiological control of food intake for the animal[J].Feed Industry,2004,25(4):26-28.(in Chinese)
[28]	MEIJER A J,DUBBELHUIS P F.Amino acid signalling and the integration of metabolism[J].Biochem Biophys Res Commun,2004,313(2):397-403.
[29]	黄雅莉,周贞兵,贺文美,等.N-氨甲酰谷氨酸对三黄鸡生长性能、血液指标及血清游离氨基酸含量的影响[J].饲料工业,2017,38(4):49-54.HUANG Y L,ZHOU Z B,HE W M,et al.Effects of N-carbamylglutamate on growth performance,blood parameters and serum free amino acid content of three yellow chicken[J].Feed Industry,2017,38(4):49-54.(in Chinese)
[30]	张艺邀.日粮可发酵有机物来源和粒度对泌乳奶牛瘤胃pH和生产性能的影响[D].泰安:山东农业大学,2018.ZHANG Y Y.The effects of fermenting organic matter source and length on the ruminal pH and performance in dairy cows[D].Taian:Shandong Agricultural University,2018.(in Chinese)
[31]	OBA M,BALDWIN R L,OWENS S L,et al.Metabolic fates of ammonia-N in ruminal epithelial and duodenal mucosal cells isolated from growing sheep[J].J Dairy Sci,2005,88(11):3963-3970.
[32]	刘建新.N-甲酰谷氨酸作为精氨酸增强剂的评定及其对高产奶牛瘤胃发酵、泌乳性能和氮利用的影响[D].杭州:浙江大学,2013.LIU J X.Evaluation of N-carbamovlglutamate as arginineenhancer and its effect on rumen fermentation,lactation performance and nitrogen utilization in high yielding lactating dairy cows[D].Hangzhou:Zhejiang University,2013.(in Chinese)
[33]	李旺.瘤胃挥发性脂肪酸的作用及影响因素[J].中国畜牧杂志,2012,48(7):63-66.LI W.The effect and influence factors of rumen volatile fatty acid[J].Chinese Journal of Animal Science,2012,48(7):63-66.(in Chinese)
[34]	赖玉娇,何烽杰,刘影,等.NCG对育肥猪生长性能的影响[J].中国畜牧业,2017(3):51-52.LAI Y J,HE F J,LIU Y,et al.N-Carbamylglutamate affects growth performance in finishing pigs[J].China Animal Industry,2017(3):51-52.(in Chinese)
[35]	李越.精氨酸琥珀酸合成酶(ASS1)对人肝癌细胞系SMMC-7721细胞增殖和凋亡的影响[D].郑州:郑州大学,2017.LI Y.Effects of Argininosuccinate synthase 1 on the proliferation and apoptosis in SMMC-7721[D].Zhengzhou:Zhengzhou University,2017.(in Chinese)
[36]	ZHOU Y W,MCSWEENEY C S,WANG J K,et al.Effects of disodium fumarate on ruminal fermentation and microbial communities in sheep fed on high-forage diets[J].Animal,2012,6(5):815-823.
[37]	朱伟云.反刍动物瘤胃甲烷生成机理及其营养调控[C]//生态环境与畜牧业可持续发展学术研讨会暨中国畜牧兽医学会2012年学术年会和第七届全国畜牧兽医青年科技工作者学术研讨会会议——特邀报告.北京:中国畜牧兽医学会,中国农业工程学会,2012:25-27.ZHU W Y.Rumen methane production mechanism and nutritional regulation[C]//Ecological Symposium on Sustainable Development of Animal Husbandry and the 2012 Annual Conference of the Chinese Society of Animal Science and Veterinary Medicine——Invited report.Beijing:Chinese Association of Animal Science and Veterinary Medicine,Chinese Society of Agricultural Engineering,2012:25-27.(in Chinese)
[38]	贺永惠,王清华,李杰.降低反刍动物甲烷排放的研究进展[J].黄牛杂志,2001,27(5):47-50.HE Y H,WANG Q H,LI J.Advance in strategies of decrease methane emission in ruminant[J].Journal of Yellow Cattle Science,2001,27(5):47-50.(in Chinese)
[39]	赵玉华,杨瑞红,王加启.瘤胃微生物甲烷生成的机理与调控[J].微生物学杂志,2005,25(5):68-73.ZHAO Y H,YANG R H,WANG J Q.Methane production mechanism and regulation of rumen microbes[J].Journal of Microbiology,2005,25(5):68-73.(in Chinese)
[40]	PAL K,PATRA A K,SAHOO A,et al.Effect of nitrate and fumarate in Prosopis cineraria and Ailanthus excelsa leaves-based diets on methane production and rumen fermentation[J].Small Rumin Res,2014,121(2-3):168-174.
[41]	杨秀清,吴瑞薇,韩作颖,等.基于mcrA基因的沁水盆地煤层气田产甲烷菌群与途径分析[J].微生物学通报,2017,44(4):795-806.YANG X Q,WU R W,HAN Z Y,et al.Analysis of methanogenic community and pathway of coalbed methane fields in the Qinshui Basin based on mcrA gene[J].Microbiology China,2017,44(4):795-806.(in Chinese)
[42]	DANIELSSON R,SCHNÜRER A,ARTHURSON V,et al.Methanogenic population and CH4 production in Swedish dairy cows fed different levels of forage[J].Appl Environ Microbiol,2012,78(17):6172-6179.
[43]	郭添福.生猪和家禽饲料中添加氨基酸提高畜牧业可持续性并减少温室气体[J].江西饲料,2013(6):12-16.GUO T F.Greenhouse gas reduction and improved sustainability of animal husbandry using amino acids in swine,poultry feeds[J].Jiangxi Feed,2013(6):12-16.(in Chinese)
[44]	吕景智.精氨酸和菊粉对肉兔肠道形态、盲肠菌群结构和生产性能的影响[D].重庆:西南大学,2017.LV J Z.Effect of arginine and inulin on intestinal morphology,caecal microflora and growth performace of meat rabbits[D].Chongqing:Southwest University,2017.(in Chinese)

Effects of Dietary Addition of N-carbamylglutamate on Growth Performance, Rumen Fermentation， Rumen Microflora and Methane Emission of Holstein Bulls

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