金龟子绿僵菌发酵液中苦马豆素含量及催化酶基因mRNA表达分析

doi:10.11843/j.issn.0366-6964.2020.04.025

Abstract

Abstract: The aim of this study was to investigate the relationship between the content of swainsonine and the mRNA expression of catalytic enzyme gene related to the biosynthesis of swainsonine in the fermentation broth of Metarhizium anisopliae. Metarhizium anisopliae was inoculated and fermented in Czapek-Dox Medium, and then we determined the swainsonine content of fermentation broth by using Q Exactive high-resolution mass spectrometer and detected the expression of mRNA of catalytic enzyme genes, namely SwnA, SwnH₁, SwnH₂, SwnK, SwnN, SwnR, and SwnT by qRT-PCR method on the 1st, 3rd, 5th and 7th day of fermentation. The results showed that the regression equation calculated from the changes in the mass concentration and peak area of swainsonine was y=31 302.5x-45 910.5 (R²=0.996 7). The highest content of swainsonine in the fermentation broth on the 3rd day was 174.014 μg·mg^-1, the mRNA expression of SwnA, SwnH₁, SwnH₂, SwnK, SwnN, SwnR, and SwnT genes have great difference in different fermentation periods. Among them, the SwnR mRNA expression was consistent with the change of swainsonine content in the fermentation broth, and the SwnH2mRNA expression was opposite to the change of swainsonine content in the fermentation broth. The results indicated that the SwnR mRNA expression was closely related to synthesis of swainsonine in Metarhizium anisopliae, which would provide an important reference for exploring fermentation process and biosynthetic pathway of swainsonine in future.

Key words: metarhizium anisopliae, swainsonine, catalyzing enzyme genes, biosynthetic pathway

CLC Number:

S182

ZHANG Yu, ZHU Yanli, LI Bo, SUN Lu, SONG Runjie, HUANG Enxia, ZHAO Baoyu, LU Hao. mRNA Expression Analysis of Catalytic Enzyme Gene and Content of Swainsonine in the Fermentation Broth of Metarhizium anisopliae[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(4): 881-887.

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mRNA Expression Analysis of Catalytic Enzyme Gene and Content of Swainsonine in the Fermentation Broth of Metarhizium anisopliae

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