ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2015, Vol. 46 ›› Issue (7): 1163-1168.doi: 10.11843/j.issn.0366-6964.2015.07.012

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Effect of RNAi on MC1R Expression and Melanin Synthesis in the Melanocytes of Alpaca

SUN Xiao-wei,ZHANG Xue-lian,PANG Quan-hai*,LI Zhen,ZHAO Li-jun,CHEN Juan-juan,NIU Feng   

  1. (College of Animal Science and Veterinary Medicine,Shanxi Agricultural University,Taigu 030801,China)
  • Received:2014-12-19 Online:2015-07-23 Published:2015-07-23

Abstract:

The objectives of this study were to evaluate the effects of RNAi on MC1R expression and melanin synthesis in the melanocytes of alpaca.Three pairs of specific siRNA for MC1R gene were designed for RNA interference in the melanocytes of alpaca.The expression of MC1R siRNA was detected by using qRT-PCR technology.The location of MC1R was detected by immunohistochemistry.The amount of melanin synthesis in melanocytes was detected by the absorbance value.The results showed that the relative expression of MC1R mRNA in the siRNA2 and siRNA3 groups was significantly lower (P<0.01) than that in the blank control group,but there were no significant differences at the relative expression of MC1R mRNA in the siRNA1 and negative control group (P>0.05);the production of melanin synthesis of siRNA2 and siRNA3 groups were significantly lower than that in the blank control group (P<0.01).The study was successfully transfected MC1R siRNA into alpaca melanocytes and the effective MC1R siRNA was screened from 3 groups of specific siRNAs.The inhibition of MC1R is consistent with the amount of melanin synthesis.MC1R of melanocytes played a regulatory role in the synthesis of melanin,and the mechanism need further research.

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