ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2015, Vol. 46 ›› Issue (4): 555-560.doi: 10.11843/j.issn.0366-6964.2015.04.007

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The Construction of Different Muscle-specific Promoter IGF2 Expression Vector and Its Impact on Bovine Skeletal Muscle Satellite Cell Proliferation

SUN Xiao-li,LI Shu-feng,TONG Hui-li,ZHANG Wei-wei,YIN Hong-yan,YAN Yun-qin*   

  1. (College of Life Science,Northeast Agricultural University,Harbin 150030,China)
  • Received:2014-07-01 Online:2015-04-23 Published:2015-04-23

Abstract:

The aim of this study was to construct IGF2 expression vector with efficient and specific promoter and study its effects on bovine skeletal muscle satellite cell proliferation.The 3 eukaryotic expression vectors containing IGF2 and muscle-specific promoter were transfected into bovine skeletal muscle satellite cells and fetal fibroblasts,the relative expression of IGF2 was detected by RT-PCR to screen efficient and specific vector.EdU experiments were carried out and RT-PCR was used to detect the cell cycle-related genes.The different promoter (desminpro,CMV-MyoGpro and MyoGpro-double) IGF2 expression vectors were constructed successfully.The test results of RT-PCR showed that pGL3-desminpro-IGF2 was an efficient and specific vector.The vector transfected into cultured cell in vitro increased the speed of cell proliferation and up-regulated the expression of cell cycle-related genes,CDK6 and IGF2.The result has laid an important foundation for the production of transgenic bovine.

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