变异黄芪内生真菌种群分布特征

doi:10.11843/j.issn.0366-6964.2014.12.021

Abstract

Abstract:

Endophyte were isolated from Astragalus variabilis samples from Tengeli of Inner Mongolia to investigate whether endophyte exist in this plant and to assess the diversity and distribution of endophyte.Surface sterilization of plant materials was taken to isolate and purify the endophyte，and species and genus of endophyte were identified by the traditional morphology and rDNA-ITS sequence analysis.The result showed that there were many kinds of endophyte in the Astragalus variabilis samples collected in Tengeli of Inner Mongolia.Under all the conditions of sterilization，the isolation rates of leaf was highest (110.00%)，and the flower was lowest (45.00%).321 strains of endophyte were isolated from 3 different tissues of Astragalus variabilis，which belonged to 17 genera，11 families，7 orders，and 5 classes.Undifilum oxytropis was the dominant species in this plant with the relative frequency 47.98%，followed with Xylaria sp.(16.51%).There was an obviously difference in quantity，species and distribution of the endophyte between different parts of Astragalus variabilis.Leaf was the most vulnerable to infection and colonization by the endophyte，and the diversity was the maximum in stem of Astragalus variabilis.

CLC Number:

S859.87

CAO Dan-dan，LU Hao，ZHAO Bao-yu，XUE Rui-xu，QUAN Hai-yun，YANG Xiao-wen，LIU Xiao-xue，YAN Du-jian. The Species Distribution Characteristics of Endophyte in Astragalus variabilis[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(12): 2057-2066.

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References

［1］周启武，赵宝玉，路浩，等.中国西部天然草地疯草生态及动物疯草中毒研究与防控现状［J］.中国农业科学，2013，46（6）：1280-1296.
［2］张有辉，潘发利，杨万科，等.巴彦洪格日苏木羊变异黄芪中毒病的调查及防治［J］.动物医学进展，2005，26（4）：118.
［3］赵宝玉，童德文，葛鹏斌，等.我国西部草原疯草危害调查［J］.中国草地，2003，25（4）：65-68.
［4］王生奎，安清聪，李志祥，等.家兔及绵羊变异黄芪中毒的病理形态学观察［J］.甘肃畜牧兽医，1998，28（4）：14-15.
［5］姚静雯，冯欢，袁玉川，等.草原毒草变异黄芪的研究进展［J］.中国草地学报，2013，35（3）：110-115.
［6］COOK D，GRUM D S，GARDNER D R，et al.Influence of endophyte genotype on swainsonine concentrations in Oxytropis sericea［J/OL］.Toxicon，2013，61：105-111.［2014-9-22］.http://ac.els-cdn.com/S0041010112007854/1-s2.0-S0041010112007854-main.pdf?_tid=2b0bcbcc-4236-11e4-8e3e-00000aab0f26&acdnat=1411376294_92e72b5099f4f636f01bac84b863eccb.
［7］GRUM D S，COOK D，GARDNER D R，et al.Influence of seed endophyte amounts on swainsonine concentrations in Astragalus and Oxytropis locoweeds［J］.J Agric Food Chem，2012，60（33）：8083-8089.
［8］COOK D，GARDNER D R，GRUM D，et al.Swainsonine and endophyte relationships in Astragalus mollissimus and Astragalus lentiginosu［J］. J Agric Food Chem，2011，59（4）：1281-1287.
［9］郭良栋.内生真菌研究进展［J］.菌物系统，2001，20（1）：148-152.
［10］文才艺，吴元华，田秀玲.植物内生菌研究进展及其存在的问题［J］.生态学杂志，2004，23（2）：86-91.
［11］孙剑秋，郭良栋，臧威，等.药用植物内生真菌及活性物质多样性研究进展［J］.西北植物学报，2006，26（7）：1505-1519.
［12］路浩，马尧，赵宝玉，等.急弯棘豆内生真菌的分离与鉴定［J］.畜牧兽医学报，2010，41（7）：902-908.
［13］余永涛，王建华.产苦马豆素疯草内生真菌的分离鉴定及其遗传多态性研究［J］.动物毒物学，2009，24（1）：36-37.
［14］BRAUN K，ROMERO J，LIDDELL C，et al.Production of swainsonine by fungal endophytes of locoweed［J］.Mycol Res，2003，107（8）：980-988.
［15］周启武，赵宝玉，路浩，等.苦马豆内生真菌分离鉴定与多样性分析［J］.畜牧兽医学报，2013，44(3)：465-474.
［16］何佳，刘笑洁，赵启美，等.植物内生真菌分离方法的研究［J］.食品科学，2009，30（15）：180-183.
［17］张素轩，邵力平，沈瑞祥.真菌分类学［M］.北京：中国林业出版社，1996.
［18］魏景超.真菌鉴定手册［M］.上海：上海科学技术出版社，1979.
［19］马尧.产苦马豆素真菌的分离鉴定及发酵条件优化［D］.杨凌：西北农林科技大学，2010.
［20］Hoveland C S.Importance and economic significance of the Acremonium endophytes to performance of animals and grass plant［J］.Agric Ecosyst Environ，1993，44（1）：3-12.
［21］王磊，章卫民，潘清灵，等.白木香内生真菌的分离及分子鉴定［J］.菌物研究，2009，7（1）：42.
［22］BAUCOM D L，ROMERO M，BELFON R，et al.Two new species of Undifilum，fungal endophytes of Astragalus (locoweeds) in the United States［J］.Botany，2012，90（9）：866-875.
［23］陈基萍，赵宝玉，路浩，等.中国主要疯草内生真菌分离鉴定及其抑菌活性研究［J］.畜牧兽医学报，2012，43（9）：1471-1478.

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The Species Distribution Characteristics of Endophyte in Astragalus variabilis

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