ELISA与IFA用于检测J亚型禽白血病病毒的比较分析

doi:10.11843/j.issn.0366-6964.2013.09.024

Abstract

Abstract:

For comparing ELISA and IFA in the detection of J subgroup avian leukosis virus, five different dilution of NX0101 TCID₅₀ virus were inoculated to DF1 cell and cultured. In six different stages (1, 2, 3, 5, 7 and 9 days culturation), ELISA was applied to detect P27 antigen within supernatant and IFA was conducted to detect the infected cells respectively. Using more than 10^1.625TCID₅₀ of the inoculation dose, IFA achieves identification of all the infected cells at 2 days and 3 days post inoculation, but the results from ELISA is relatively lag of 2-4 days for confirmation. Inoculating less than 10^1.625TCID₅₀ of virus, IFA detect successfully the infected cells while ELISA still not detect by 7th day. The results suggest that IFA is more accurate and effective than commercial ELISA kit, ELISA have obvious hysteresis in comparison to IFA.

CLC Number:

S854.43

WANG Xin, CUI Zhi-zhong, GONG Jian-sen, GAO Ming-yan, FAN Jian-hua,YU Yan, ZHU Jing, XU Bu. Comparison of ELISA and IFA in the Detection of the J Subgroup Avian Leukosis Virus[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2013, 44(9): 1499-1503.

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References

［1］PAYNE L N, FADLY A M. Leukosis/Sarcoma group ［M］//Diseases of Poultry. Calnek B W. Towa State University Press, Ames, USA, 1997, 414-466.

［2］PAYNE L N. HPRS-103: a retrovirus strikes back. The emergence of subgroup J avian leukosis virus［J］. Avian Pathol, 1998, 27(Supp. 1): S36-S45.

［3］PAYNE L N, HOWES K, GILLESPIE A M. Host range of Rous sarcoma virus pseudo type RSV (HPRS-103) in 12 avians peces:support for a new avian retrovirus envelop subgroup, designed J ［J］. J Gen Virol, 1992, 73(11):2995-2997.

［4］杜岩，崔治中，秦爱建.从市场商品肉鸡中检出J亚群禽白血病病毒［J］.中国家禽, 1999(1):1-4.

［5］徐镔蕊，董卫星，余春明，等.用ALV-J gp85单克隆抗体证明蛋鸡存在J亚群禽白血病［J］.畜牧兽医学报, 2005, 36(3):269-271.

［6］成子强，张利，刘思当，等.中国麻鸡中发现禽J亚群白血病［J］.微生物学报，2005，45(4):584-587.

［7］SUN S, CUI Z. Epidemiological and pathological studies of subgroup J avian leukosis virus infections in Chinese local "yellow"chickens ［J］. Avian Pathol, 2007, 36(3): 221-226.

［8］王鑫，李德庆，边小明，等. 海兰褐产蛋鸡ALV-J亚型相关纤维肉瘤的鉴别诊断及人工造病试验［J］.中国兽医科学,2012,42(6):582-586.

［9］秦爱建，刘岳龙，周绮雯，等.J亚群禽白血病病毒的免疫荧光检测效果［J］.中国预防兽医学报,2001,23(3):214-216.

［10］秦爱建，崔治中，LEE L，等.抗J亚群禽白血病病毒囊膜糖蛋白特异性单克隆抗体的研制及其特性［J］.畜牧兽医学报，2001，32(6)：556-562.

［11］DONG X, LIU J, LI D Q, et al. Comparison of IFA and ELISA in the detection of avain leukosis virus subgroup J in DF-1 cell cultures ［J］. Inter J Vet Sci, 2012, 1(1):13-15.

［12］张志，赵宏坤，崔治中.宁夏肉用种鸡J亚群禽白血病的实验室诊断［J］.中国兽医科技, 2002, 32(11)：25-26.

［13］崔治中.鸡白血病及其鉴别诊断和预防控制［J］.中国家禽，2010，32(8):1-12.

［14］郭慧君，李中明，李宏梅，等.3种ELISA 试剂盒检测不同亚型外源性鸡白血病病毒的比较［J］.畜牧兽医学报，2010，41(3):310-314.

［15］李薛，李德庆，赵鹏，等.ELISA与IFA检测鸡血清ALV-A/B特异性抗体相关性研究［J］. 病毒学报，2012，28(6):615-620.

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Comparison of ELISA and IFA in the Detection of the J Subgroup Avian Leukosis Virus

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