柔嫩艾美耳球虫入侵相关蛋白EtMA1和EtMA2的克隆及序列分析

doi:10.11843/j.issn.0366-6964.2013.11.018

Abstract

Abstract:

To screen the novel candidate vaccine for E. tenella, the genes of EtMA1 and EtMA2 associated with parasites invasion were cloned and the sequences were analyzed. The sequence of EtAMA1 cloned in our laboratory were analyzed by the bioinformatics tool and the technology of comparative genomics, and the gene sequences encoding EtMA1 and EtMA2 were acquared. The EtMA1 and EtMA2 ORF were amplified by RT-PCR from E. tenella (Guangdong strain) merozoites total RNA template. The sequence structures of EtMA1 and EtMA2, and the evolution relationship with other apicomplexan parasites were analyzed by bioinformatics software on line. The result showed that the ORF of EtMA1 and EtMA2 were 1 617 and 1 725 bp in full length, and coding the peptides of 549 and 575 aa in length. The amino acid sequences were highly conserved with E. tenella apical membrane antigen (EtAMA1) and TgAMA1. Both of the EtMA1 and EtMA2 were superfamily proteins of AMA, and which maybe play a key role in parasites invasion of host cells, and both of which will be as novel candidate vaccine for E. tenella.

CLC Number:

S852.723

QI Nan-shan, CHEN Hai-ning, LYU Min-na, WU Cai-yan, LIAO Shen-quan, LI Juan, TONG Zong-xi, SUN Ming-fei. Cloning and Sequence Analysis of Eimeria tenella Invasion-associated Proteins, EtMA1 and EtMA2[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2013, 44(11): 1826-1831.

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Cloning and Sequence Analysis of Eimeria tenella Invasion-associated Proteins, EtMA1 and EtMA2

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