ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2012, Vol. 43 ›› Issue (9): 1499-1503.doi:

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Screening and Analysis of Differential Expressed Sequence Tags in Skin Hair
Follicles for Cashmere Goats

SU Li-ning1,2,3, LI Hua1,2, LIU Dong-jun1, XU Ri-gan1*   

  1. (1. The Key Laboratory for Mammalian Reproductive Biology and Biotechnology of Ministry
     of Education, Inner Mongolia University, Hohhot 010021, China; 2. College of Life Science,
     Foshan University, Foshan 528231, China; 3. Hebei North University,Zhangjiakou 075000, China)
  • Received:2011-12-21 Online:2012-09-25 Published:2012-09-25

Abstract: To screen and analyze the genes associated with growth of goats’ wool and cashmere, differential display reverse transcription polymerase chain reaction (DDRT-PCR) technology was used to study the differentially expressed sequence tags ( ESTs ) at different embryo ages in skin primary and secondary follicles of Inner Mongolia Cashmere goats. 15 ESTs were identified (Acc_N: JK711022-JK711036) , of which 3 ESTs might play a role in the development of hair follicle, and 5 ESTs might be related to secondary follicles and sebaceous glands. The results of bioinformatics analysis showed that JK711036 shared homology with other species’ TRIP12 gene, but the function of other ESTs were unknown. All these were showed that many differential expressed genes was associated with primary and secondary wool follicles on metabolism pathways, which would establish molecular foundation for the formation of wool and cashmere in goats.

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