成年版纳微型猪近交系克隆猪的制备

Abstract

Abstract: Fibroblast cells were isolated from adult Banna Miniature Inbred pig with cold trypsin digestion method and subcultured. Through study of cell biology characteristics, effects of fibroblast cells as the donor cells on somatic cell nuclear transfer was investigated. The results showed that isolated fibroblast cells were morphologically consistent with typical fibroblast, cell viability was 97.2% before freezing and 92.6% after thawing (P>0.05). The cell growth curve was ‘S’ shape and population doubling time was approximately 36 h. The karyotype was normal after 14 passages of subculture in vitro. When isolated fibroblast cells were used as donor cells for somatic cell nuclear transfer, the cleavage rate, the blastocyst rate and cell number of blastocyst were 61.9%, 13.0% and 37, respectively. One of three surrogate pigs after somatic cell nuclear transfer with fibroblast cells as donor cells was pregnant and gave birth to one clone pig. In brief, fibroblast cell line could be established from adult Banna Miniature Inbred pig. The isolated fibroblast cells could be used as donor cells for somatic cell nuclear transfer and gave birth to the clone pig of Banna Miniature Inbred pig.

CLC Number:

S828
S813.3

YE Lei, LI Hong, WEI Hong-Jiang, XU Cheng-Sheng, QING Yu-Bo, PAN Wei-Rong, LI Hong-Hui, CHENG Wen-Min. Production of Cloning Adult Banna Miniature Inbred Pig[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2012, 43(9): 1491-1498.

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Production of Cloning Adult Banna Miniature Inbred Pig

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