Abstract: To identify the Torque teno sus virus (TTSuV), a PCR-DHPLC assay was developed in this study. Primers specific for the partial region of the TTSuV1 and TTSuV2 were selected to conduct the PCR-DHPLC assays. The specificity test was performed with PPV, PCV-Ⅱ and PRV and no cross reaction was found. The PCR-DHPLC for TTSuV had good specificity and nice repeatability. Sensitivity analysis showed that the developed PCR-DHPLC could detect 1.0×10¹ copy·μL^－1. The established method and the Real-time PCR were used to detect 80 serum samples, the results showed that 63 samples were TTSuV1 positive by PCR-DHPLC, and it was consistent with real-time PCR test; 69 samples were TTSuV2 positive by PCR-DHPLC, and it was consistent with real-time PCR test, 56 samples were positive by normal RT-PCR. The method showed nice specification, sensitivity, repeatability, and quickness, and it could be used in epidemiological investigation of TTSuV.