ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2012, Vol. 43 ›› Issue (9): 1415-1421.doi:

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Establishment of Judging Standard of Colloidal Gold-based Immunochromatographic
Assay Strip for Detection Antibodes of Serotype O Foot-and-mouth Disease

ZHI Xiao-ying1,2, REN Wei-wei1,2, QI Guang-yu1,2, LV Jian-liang1, LIANG Zhong1, JIANG Tao1*   

  1. (1. Key Laboratory of Animal Virology of Ministry of Agriculture, State Key Laboratory of
    Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of
    Agricultural Sciences, Lanzhou 730046, China; 2. China Agricultural Vet.Bio.Science and
    Technology Co.,Ltd., Lanzhou 730046, China; 3. The First Clinical Medicine College of
    Lanzhou University, Lanzhou 730000, China)
  • Received:2012-01-04 Online:2012-09-25 Published:2012-09-25

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Abstract: The objective of the study was to establish a judging standard of colloidal gold-based immunochromatographic assay strip (GICA) for detecting antibodies of serotype O of foot-and-mouth disease (FMD) rapidly. The GICA of 3 batches were tested. There were a hundred and eighty FMD negative serum samples (containing pig, cattle and sheep), a hundred and ninety-seven FMD weak positive serum samples (containing pig, cattle), three hundred and seventeen FMD serotype O positive serum samples (containing pig, cattle and sheep), two hundred and twenty-three FMD serotype A and Asia 1 positive cattle sera, six hundred filed samples. To compare three methods of GICA, liquid-phase competition ELISA (LB-ELISA) of FMDV serotype O and Indirect Hemagglutination Test (IHA) of FMDV serotype O for detecting antibodies of FMD serotype O, all the serum samples were detected, the judging standard was established. According to the color of test line and control line, compared with the titers of LB-ELISA, the colorimetric card was drawn. The judging standard of GICA was as follows: Using a 1:2 dilution of the sample, the result was negative, and the anti-FMDV serum was negative; Using a 1:8 dilution of the sample, the result was positive, which implied that the serum had serotype O FMDV positive antibodies. Compared with GICA and LB-ELISA, the colorimetric card was drawn which was helpful for reading results. The correlation of the GICA and LPBE was y = 1.142x + 0.742 1; the correlation coefficient (r) was 0.922 1; the correlation of the GICA and IHA was y = 1.184 5x + 0.862 3; the correlation coefficient (r) was 0.903 3. These results indicated that the judging standard of GICA was prior to detecting antibodies of FMD serotype O; the colorimetric card was easy to read, which was just monitoring the anti-FMD serotype O antibodies of immunized animals fast and accurately. This judging standard could promote the qualitative analysis to semi-quantitative analysis, and it had provided that it was more suitable for using in laboratory and the field.

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