ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2012, Vol. 43 ›› Issue (6): 909-914.doi:

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Molecular Diversity of Rumen Methanogens from the Goat and Its Comparison with Other Ruminants

PEI Cai-xia1,2, MAO Sheng-yong1, ZHU Wei-yun1*   

  1. 1. Laboratory of Gastrointestinal Microbiology, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China; 2. College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu 030801, China
  • Received:2011-05-30 Revised:1900-01-01 Online:2012-06-25 Published:2012-06-25
  • Contact: ZHU Weiyun

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Abstract:

This experiment was conducted to study the molecular diversity of rumen methanogens from goat and its comparison with other ruminant. The methanogenspecific primers Met86F/Met1340R was used to analyze the 16S rRNA gene diversity of methanogens. One hundred clones were randomly picked up for sequence analysis. The result showed that sixteen OTUs were obtained after RFLP analysis, and the majority belonged to the Methanobrevibacter strains, AK-87 (58%), and others to ZA-10 (19%), OCP (7%), SM9 (2%), and 30Y(2%), and Methanosphaera stadtmanae-like (1%), Methanobacterium aarhusense-like (2%), Aciduliprofundum boonei-like (3%), Methanobrevibacter sp. AK-87-like (4%) and Methanobrevibacter sp. 1Y-like (2%) sequences, respectively. With comparison to rumen methanogens from other ruminants reported in previous studies, it can be concluded that the structure of methanogenic communities in the rumen can be affected by the different primers, feed types and animal species.

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