ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2008, Vol. 39 ›› Issue (10): 1402-1405.doi:

• 预防兽医 • Previous Articles     Next Articles

Expression of 10 ku Protein Gene from Taenia solium and Its Preliminary Application

WU Guo-hua, ZHENG Ya-dong, JIA Wan-zhong, ZHANG Shao-hua,
JING Zhi-zhong, CAI Xue-peng*   

  1. Key Laboratory of Zoonoses of Veterinary Public Health of Ministry of Agriculture, Key Laboratory of Veterinary Parasitology of Gansu Province, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2008-10-24 Published:2008-10-24

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Abstract: Recombinant expression plasmids, designated as pGEX-4T-TSO10 and pGEX-4T-CE10, were constructed by insertion of the TSO10 gene from Taenia solium oncosphere and the CE10 gene from the larva, respectively, and identified by sequencing. The results of SDS-PAGE, Western-blot and lamellar scanning showed that both fusion target proteins expressed in E. coli were 35 ku in molecular weight and can be recognized by the positive serum from a cysticercosis patient, and that their contents accounted for 25% and 30% of total bacteria proteins, respectively. Furthermore, the results of ELISA indicated that the recombinant proteins are suitable for diagnosis of cysticercosis.