Abstract: With reverse transcription polymerase chain reaction(RT-PCR), P27/30 gene was cloned from parthenogenetic Haemaphysalis longicornis of China. The length of P27/30 gene was 670 bp, it included complete ORF and encoded 201 amino acid residues，the deduced mass was 23.38 kDa．Its nucleotide sequence exhibited 99.85％ similarity to that of the P27/30 gene from Japan parthenogenetic Haemaphysalis longicornis. Results of RT-PCR analysis showed that the P27/30 gene could express in the eggs，larvae，nymphs，fame adult ticks and engorgement adult ticks．After subcloned into expressing vector pET32a（+），it could express in E．coli BL21(DE3) . The recombinant protein was about 24 kDa．With Western-blot analysis, the recombination express protein was approved to be able to recognize antibody of Haemaphysalis longicornis.