Abstract: Total RNA was extracted from the tougue epithelia of a reindeer and the cDNA encoding reindeer β-defensin (reBD-1) was amplified by the reverse transcription PCR(RT-PCR).The purified RT-PCR product was cloned in pBlueselect T vector. The results of restriction endonuclease pattern analysis of recombinant plasmid and DNA sequencing demonstrated that the reBD-1 is belong to the family of β-defensin because the cDNA contain a open reading frame (ORF) of 192 bases which encoded a 64 amino acid prepro-peptide and the prepro-peptide contained the β-defensin consensus sequence of six invariantly spaced cysteine residues. The reBD-1 is the first β-defensin found in reindeers. This finding of the reindeer β-defensin facilitate a better understanding of mucosal defense mechanisms in reindeers.