基于体细胞克隆的超级公猪快速扩繁技术的效果评价分析

doi:10.11843/j.issn.0366-6964.2025.12.022

Abstract

Abstract: The study aimed to evaluate the application effect of somatic cell cloning technology (SCNT) in the rapid expansion of Topigs E line super boars. Using 12 super boars as donors, 1 100 embryos were isolated, cultured, frozen, amplified and subcultured, and transferred to 6 sows. Finally, 4 sows delivered 29 piglets (cloning efficiency 2.63%). The growth and develo-pment, semen quality and reproductive performance of cloned and non-cloned pigs were compared and analyzed. The results showed that there was no significant difference in growth and development between cloned pigs and donor normal offspring. There was no significant difference in semen volume ((188.17±49.27) mL) and effective sperm number ((486.35±159.56) million) between cloned pigs and donor siblings and donor half-sibs. There was no significant difference in reproductive performance of sows after mating. SNP detection showed that the inconsistency rate between cloned pigs and donor genes was below 0.14%, indicating that genetic information was derived from donors. In summary, there was no significant difference in reproductive performance and growth performance between cloned pigs and donor offspring. SCNT technology can be effectively used for super boar expansion production.

Key words: somatic cell cloning, breeding boars, expanding reproduction

CLC Number:

S828.3

LIU Bin, ZHU Kangping, ZHOU Chengpeng, ZHOU Lixin, XUE Jia, SHEN Linyuan, GAN Mailin, ZHU Li. Analysis on the Effect of Rapid Population Expansion of Excellent Boars Based on Somatic Cell Cloning[J]. Acta Veterinaria et Zootechnica Sinica, 2025, 56(12): 6191-6203.

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Analysis on the Effect of Rapid Population Expansion of Excellent Boars Based on Somatic Cell Cloning

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