基于全转录组测序的2个不同品种绵羊卵巢ceRNA网络构建及关键miRNA的筛选

doi:10.11843/j.issn.0366-6964.2025.06.021

Abstract

Abstract:

This study aimed to analyze the molecular regulation mechanism of ncRNA (non-coding RNA) on the development process of sheep ovary, and screen the key genes for improving sheep fertility. In this study, 3 healthy adult ewes were selected from multiparous sheep (Small-tail Han sheep) and single-born sheep (Ujumuqin sheep) to construct ncRNA and mRNA expression profiles of ovarian tissue, the differentially expressed lncRNAs, circRNAs, miRNAs and mRNAs were screened, and their target genes were predicted and GO and KEGG enrichment analysis were performed. Reproductive related pathways were screened out to further construct the ceRNA (lncRNA-miRNA-mRNA and circRNA-miRNA-mRNA) regulatory network, and finally the key miRNAs in the regulatory network were summarized. The results showed that a total of 1 579 lncRNAs, 561 circRNAs, 175 miRNAs and 3 095 mRNAs differentially expressed in the ovarian tissues of Ujumuqin sheep and small-tailed Han sheep were screened. The results of GO and KEGG enrichment analysis showed that the target genes were significantly enriched in biological processes such as embryonic morphogenesis, meiosis activation, and mucin type O-glycan biosynthesis, glycosphingolipid biosynthesis, D-arginine and D-ornithine metabolism and other signaling pathways. The differentially expressed genes enriched in reproductive pathways were screened, and a ceRNA network(contained 87 lncRNAs, 27 circiRNAs, 3 miRNAs and 3 mRNAs)was constructed to obtain three key miRNAs(miR-140, miR-338 and miR-423-5p)and corresponding targeted regulatory ceRNA networks. Real-time fluorescence quantification (qRT-PCR) detection results of 9 differentially expressed lncRNAs, circRNAs, miRNAs, mRNAs, randomly selected were consistent with the trend of high-throughput sequencing results, indicating the reliability of sequencing data. This study analyzed the coordinated regulatory mechanism of DE ncRNAs and corresponding target genes, which provided an effective theoretical basis for elucidating the key genes and molecular mechanisms regulating the development of sheep ovary, and further fully understood the reproductive biological characteristics of sheep, which has important guiding significance for improving the reproductive efficiency.

Key words: Ujumuqin sheep, Small-tailed Han sheep, ovary, lncRNA, circRNA, miRNA, ceRNA network

CLC Number:

S826.3

GU Bo, WANG Anqi, YU Xinmiao, GUO Juntong, YANG Yi, DENG Yijie, JIANG Huaizhi. Construction of Ovarian ceRNA Networks and Screening of Key miRNA in Two Different Breeds of Sheep Based on Whole Transcription Sequencing[J]. Acta Veterinaria et Zootechnica Sinica, 2025, 56(6): 2765-2777.

Figures/Tables 11

Table 1

Table 2

Table 3

Fig. 1

Fig. 2

Fig. 3

Fig. 4

Fig. 5

Fig. 6

Fig. 7

Fig. 8

References 45

1	MA N , TIE C , YU B , et al. Identifying lncRNA-miRNA-mRNA networks to investigate Alzheimer's disease pathogenesis and therapy strategy[J]. Aging (Albany NY), 2020, 12 (3): 2897- 2920.
2	REN J , JIANG C , ZHANG H , et al. LncRNA-mediated ceRNA networks provide novel potential biomarkers for peanut drought tolerance[J]. Physiol Plant, 2022, 174 (1): e13610. doi: 10.1111/ppl.13610
3	YANG T , QIU L , YONG J , et al. Identification, biogenesis, and function prediction of a novel circRNA3238 of chicken[J]. Anim Biotechnol, 2023, 34 (7): 2527- 2536. doi: 10.1080/10495398.2022.2102504
4	牛熙. 香猪发情期卵巢lncRNA表达分析及调控网络研究[D]. 贵阳: 贵州大学, 2023.
	NIU X. Analysis of ovarian lncRNA expression and regulatory network in perfumed pigs during estrus[D]. Guiyang: Guizhou University, 2023. (in Chinese)
5	张珂, 张敏, 李添宝, 等. 广西麻鸡卵巢组织中差异lncRNA筛选及ceRNA网络构建[J]. 中国畜牧兽医, 2024, 51 (7): 2739- 2750.
	ZHANG K , ZHANG M , LI T B , et al. Screening of differential lncRNAs and construction of ceRNA network in ovary tissue of Guangxi Ma chicken[J]. China Animal Husbandry & Veterinary Medicine, 2024, 51 (7): 2739- 2750.
6	孟朝轶, 王运路, 姚一龙, 等. lncRNA-MSTRG.7889.1竞争性结合bta-miR-146a靶向Smad4调控牦牛颗粒细胞的凋亡[J]. 中国农业科学, 2024, 57 (4): 797- 809.
	MENG C Y , WANG Y L , YAO Y L , et al. lncRNA-MSTRG.7889.1 competitively binds to bta-miR-146a targeting Smad4 to regulate apoptosis of yak granulosa cells[J]. Scientia Agricultura Sinica, 2024, 57 (4): 797- 809.
7	刘在霞, 刘永斌, 石彩霞, 等. 不同繁殖力绵羊卵巢转录组比较分析[J]. 中国农业大学学报, 2023, 28 (12): 132- 143. doi: 10.11841/j.issn.1007-4333.2023.12.12
	LIU Z X , LIU Y B , SHI C X , et al. Comparative analysis of ovary transcriptome of sheep with different fecundity[J]. Journal of China Agricultural University, 2023, 28 (12): 132- 143. doi: 10.11841/j.issn.1007-4333.2023.12.12
8	SHARIATI GAZGAZAREH P , MASOUDI A , VAEZ TORSGIZI R , et al. Gene expression pattern and molecular mechanisms involved in Shal and Sangsari sheep fertility using RNA-Seq[J]. Reprod Domest Anim, 2023, 58 (4): 548- 559. doi: 10.1111/rda.14325
9	WANG C , ZHAO Y , YUAN Z , et al. Genome-wide identification of mRNAs, lncRNAs, and proteins, and their relationship with sheep fecundity[J]. Front Genet, 2022, 12, 750947. doi: 10.3389/fgene.2021.750947
10	马淑娇. 蒙古羊和乌珠穆沁羊LEPR基因的多态性及其与多胎性状的关联性分析[D]. 呼和浩特: 内蒙古大学, 2022.
	MA S J. Association between novel ploymorphisms in LEPR gene and litter size in mongolia and ujimoin sheep breeds[D]. Hohhot: Inner Mongolia Agricultural University, 2022. (in Chinese)
11	MARTIN M . Cutadapt removes adapter sequences from high-throughput sequencing reads[J]. EMBnet J, 2011, 17 (1): 10. doi: 10.14806/ej.17.1.200
12	KIM D , PAGGI J M , PARK C , et al. Graph-based genome alignment and genotyping with HISAT2 and HISAT-genotype[J]. Nat Biotechnol, 2019, 37 (8): 907- 915. doi: 10.1038/s41587-019-0201-4
13	KIM D , LANGMEAD B , SALZBERG S L . HISAT: A fast spliced aligner with low memory requirements[J]. Nat Methods, 2015, 12 (4): 357- 360. doi: 10.1038/nmeth.3317
14	PERTEA M , KIM D , PERTEA G M , et al. Transcript-level expression analysis of RNA-seq experiments with HISAT, StringTie and Ballgown[J]. Nat Protoc, 2016, 11 (9): 1650- 1667. doi: 10.1038/nprot.2016.095
15	ZHANG X , DONG R , ZHANG Y , et al. Diverse alternative back-splicing and alternative splicing landscape of circular RNAs[J]. Genome Res, 2016, 26 (9): 1277- 1287. doi: 10.1101/gr.202895.115
16	GAO Y , WANG J , ZHAO F . CIRI: An efficient and unbiased algorithm for de novo circular RNA identification[J]. Genome Biol, 2015, 16 (1): 4. doi: 10.1186/s13059-014-0571-3
17	ZHU L , LI N , SUN L , et al. Non-coding RNAs: The key detectors and regulators in cardiovascular disease[J]. Genomics, 2021, 113 (1 Pt 2): 1233- 1246.
18	YANG D , WANG Y , ZHENG Y , et al. Silencing of lncRNA UCA1 inhibited the pathological progression in PCOS mice through the regulation of PI3K/AKT signaling pathway[J]. J Ovarian Res, 2021, 14 (1): 48. doi: 10.1186/s13048-021-00792-2
19	SU T , YU H , LUO G , et al. The Interaction of lncRNA XLOC-2222497, AKR1C1, and Progesterone in Porcine Endometrium and Pregnancy[J]. Int J Mol Sci, 2020, 21 (9): 3232. doi: 10.3390/ijms21093232
20	LI J , CAO Y , XU X , et al. Increased new lncRNA-mRNA gene pair levels in human cumulus cells correlate with oocyte maturation and embryo development[J]. Reprod Sci, 2015, 22 (8): 1008- 1014. doi: 10.1177/1933719115570911
21	YERUSHALMI G M , SALMON-DIVON M , YUNG Y , et al. Characterization of the human cumulus cell transcriptome during final follicular maturation and ovulation[J]. Mol Hum Reprod, 2014, 20 (8): 719- 735. doi: 10.1093/molehr/gau031
22	岳炳霖. 牛circRNA的ceRNA网络构建及调控牛骨骼肌细胞发育的功能机制研究[D]. 杨凌: 西北农林科技大学, 2021.
	YUE B L. Construction of the ceRNA network of bovine circRNA and the functional mechanism of regulating the development of bovine skeletal muscle cells[D]. Yangling: Northwest A&F University, 2021. (in Chinese)
23	刘梦馨, 杜双杨, 杨京沧, 等. lncRNA作为竞争性内源分子的作用机制及研究进展[J]. 中国畜牧兽医, 2021, 48 (10): 3604- 3613.
	LIU M X , DU S Y , YANG J C , et al. Mechanism and research progress of lncrnas copetitive endogenous molecules[J]. China Animal Husbandry & Veterinary Medicine, 2021, 48 (10): 3604- 3613.
24	DUAN B , XU X Z S . How to break a fever: A feedback circuit for body temperature control[J]. Neuron, 2019, 103 (2): 179- 181. doi: 10.1016/j.neuron.2019.06.023
25	陈玉林, 刘玉芳, 储明星. 非编码RNA在动物繁殖中的研究进展[J]. 中国草食动物学, 2023, 43 (6): 39- 44.
	CHEN Y L , LIU Y F , CHU M X . Research Progress of Non-coding RNA in Animal Reproduction[J]. China Herbivore Science, 2023, 43 (6): 39- 44.
26	吴士良. 黏蛋白型O-聚糖: 结构、功能及与肿瘤的相关性[J]. 生命科学, 2011, 23 (6): 563- 568.
	WU S L . Mucin-type O-glycans in human cancer: structures and functions[J]. Chinese Bulletin of Life Sciences, 2011, 23 (6): 563- 568.
27	陈尔希蒂. MiR-140-3p在卵巢储备功能减退中的表达及其相关功能机制研究[D]. 杭州: 浙江大学, 2021.
	CHEN E X D. The roles and mechanisms of miR-140-3p in the diminished ovarian reserve[D]. Hangzhou: Zhejiang University, 2021. (in Chinese)
28	DUAN P , HA M , HUANG X , et al. Intronic miR-140-5p contributes to beta-cypermethrin-mediated testosterone decline[J]. Sci Total Environ, 2022, 806 (Pt.1): 10517.
29	杨俊, 祝徳, 段智慧, 等. miR-140抑制卵巢癌细胞增殖促进卵巢癌细胞凋亡及靶向抑制CMTM6表达[J]. 中国组织化学与细胞化学杂志, 2022, 31 (6): 582- 591.
	YANG J , ZHU D , DUAN Z H , et al. Inhibition of proliferation, promotion of apoptosis, and target inhibition of CMTM6 expression by miR-140 in ovarian cancer cells[J]. Chinese Journal of Histochemistry and Cytochemistry, 2022, 31 (6): 582- 591.
30	刘娇. Circ_0000231通过miR-140结合RAP1B介导卵巢癌细胞恶性生物学行为及紫杉醇耐药的机制研究[D]. 沈阳: 中国医科大学, 2022.
	LIU J. The study on mechanisms of circ_0000231 competitively binds to miR-140/RAP1B to further mediate malignant biological behavior of ovarian cancer cells and paclitaxel resistance[D]. Shenyang: China Medical University, 2022. (in Chinese)
31	吴大英, 张秀艳, 李霞, 等. 兔抗人MiR-338抗体制备与鉴定[J]. 九江学院学报(自然科学版), 2020, 35 (2): 96- 99.
	WU D Y , ZHANG X Y , LI X , et al. Preparation and identification of rabbit anti-human antibody of miR-338[J]. Journal of jiujiang University (natural sciences), 2020, 35 (2): 96- 99.
32	张瑞涛, 李松, 史惠蓉, 等. 卵巢上皮性癌细胞中miR-338-3p过表达对MACC1表达及细胞迁移和侵袭的影响[J]. 郑州大学学报(医学版), 2021, 56 (1): 32- 38.
	ZHANG R T , LI S , SHI H R , et al. Effects of miR-338-3p overexpression in epithelial ovarian cancer cells on MACC1 expression and cell migration and invasion[J]. Journal of Zhengzhou University(Medical Sciences), 2021, 56 (1): 32- 38.
33	ZHANG R , SHI H , REN F , et al. MicroRNA-338-3p suppresses ovarian cancer cells growth and metastasis: implication of Wnt/catenin beta and MEK/ERK signaling pathways[J]. J Exp Clin Cancer Res, 2024, 43 (1): 48.
34	赵凌. LncPHLPP1-miR-338-y-CCND1轴调控PI3K/AKT通路对牦牛卵泡体细胞增殖分化的影响[D]. 兰州: 甘肃农业大学, 2023.
	ZHAO L. Effect of lncPHLPP1-miR-338-y-CCND1 axis regulation PI3K/AKT pathway on proliferation and differentiation of yak follicular somatic cells[D]. Lanzhou: Gansu Agricultural University, 2023. (in Chinese)
35	徐子雯. MiR-338-3p通过影响颗粒细胞功能和卵泡发育参与卵巢储备调控的机制研究[D]. 郑州: 郑州大学, 2021.
	XU Z W. MiR-338-3p participates in the regulation of ovarian reserve by affecting granulosa cell function and follicular development[D]. Zhengzhou: Zhengzhou University, 2021. (in Chinese)
36	金雪峰, 杨广宇. 鞘糖脂与肿瘤疾病相关性研究进展[J]. 中国临床新医学, 2021, 14 (10): 976- 980.
	JIN X F , YANG G Y . Research progress in the correlation between glycosphingolipids and tumor diseases[J]. Chinese Journal of New Clinical Medicine, 2021, 14 (10): 976- 980.
37	李晓荣, 仲佳雯, 秦岭, 等. 卵巢储备功能减退和卵巢早衰模型小鼠的差异蛋白质组学研究[J]. 广东医学, 2024, 45 (1): 68- 76.
	LI X R , ZHONG J W , QIN L , et al. Differential proteomic study of ovarian reserve decline and premature ovarian insufficiency model mice[J]. Guangdong Medical Journal, 2024, 45 (1): 68- 76.
38	陈雪峰, 王春琳, 顾志敏, 等. 罗氏沼虾(Macrobrachium rosenbergii)卵巢发育不同时期转录组分析[J]. 海洋与湖沼, 2019, 50 (2): 398- 408.
	CHEN X F , WANG C L , GU Z M , et al. Transcriptome analysis of Macrobrachium rosenbergii ovary in four development stages[J]. Oceanologia Et Limnologia Sinica, 2019, 50 (2): 398- 408.
39	衣欢, 郑祥钦, 宋建榕, 等. 卵巢癌外泌体携运miRNAs调控葡萄糖代谢生物信息学分析[J]. 福建医药杂志, 2019, 41 (5): 117- 119.
	YI H , ZHENG X Q , SONG J R , et al. Bioinformatics analysis of ovarian-cancer-secreted exosomal miRNAs regulation glucose metabolism[J]. Fujian Medical Journal, 2019, 41 (5): 117- 119.
40	XU X , GUAN R , GONG K , et al. Circ_FURIN knockdown assuages Testosterone-induced human ovarian granulosa-like tumor cell disorders by sponging miR-423-5p to reduce MTM1 expression in polycystic ovary syndrome[J]. Reprod Biol Endocrinol, 2022, 20 (1): 32.
41	SUI S , JIA Y , HE B , et al. Maternal low-protein diet alters ovarian expression of folliculogenic and steroidogenic genes and their regulatory microRNAs in neonatal piglets[J]. Asian-Australas J Anim Sci, 2014, 27 (12): 1695- 1704.
42	于兰兰. 参与初情期启动的山羊卵巢miRNA筛选与鉴定[D]. 合肥: 安徽农业大学, 2016.
	YU L L. Screening and identification of ovarian miRNA involved in puberty initiation in goat[D]. Hefei: Anhui Agricultural University, 2016. (in Chinese)
43	宋洋, 任芳. miR-423-5p在卵巢癌组织中的表达及对癌细胞增殖的影响[J]. 医学临床研究, 2018, 35 (9): 1675- 1678.
	SONG Y , REN F . Effect of miR-423-5p expression on the proliferatioan ovarian cancer cells[J]. Journal of Clinical Research, 2018, 35 (9): 1675- 1678.
44	孔亚茹. 猪有腔卵泡内卵母细胞成熟的转录组分析[D]. 广州: 华南农业大学, 2020.
	KONG Y R. Transcriptome analysis of oocyte maturation in pig antrum follicles[D]. Guangzhou: South China Agricultural University, 2020. (in Chinese)
45	YEO H L , FAN T C , LIN R J , et al. Sialylation of vasorin by ST3Gal1 facilitates TGF-β1-mediated tumor angiogenesis and progression[J]. Int J Cancer, 2019, 144 (8): 1996- 2007.

目的基因 Gene name	引物序列(5′→3′) Sequence information	长度/bp Length	退火温度/℃ Annealing temperature
β-actin	F: GATCTGGCACCACACCTTCTA	115	60
	R: GATCTGGGTCATCTTCTCACG
MSTRG.7755	F: GCGTTGCTCTGTTCTTTATCTCCTG	144	60
	R: ACCTGTGCTGTCTGAGTCTTGTG
MSTRG.25228	F: CCTGATCTTGTCCATCGTCCTATCC	123	60
	R: GGGAACTGACCAAGGATGATAAGGG
MSTRG.28959	F: TGCTAAGCCTATGCCACTAGATGC	128	60
	R: CTCAAAGTCACCAGTTTCCCAAAGG
circRNA4099	F: TGGAGCCTGTAATAACATGTGAC	259	60
	R: AGTTCCGCTGTGTTTGCATC
circRNA2195	F: GGCTGGAGGTTTAAGAAGCG	125	60
	R: TTGAGGAAGGCAGACAGGTC
circRNA15689	F: GTGAGCAAATTGTGGAAGTTGGA	102	60
	R: GGCTTCAAATCTCATGCCCG
CXCR1	F: GCTGACCTGCTCTTCGCCATG	160	60
	R: GGTAGCGGTCCATGCTGATGC
POLR2I	F: AGTGGCAGGACTGGAGTTCGG	145	60
	R: GGTCTTCGGTTCGCGGCAAC
RPS20	F: CCAGCCGCAACGTGAAGTCTC	81	60
	R: GCCGACCTCGCTCAGAACAAG
oar-miR-433-3p	F: AACGGCATCATGATGGGCTCC	65	60
	R: ATCCAGTGCAGGGTCCGAGG
oar-miR-200c	F: AAGCGCCTTAATACTGCCGGG	65	60
	R: ATCCAGTGCAGGGTCCGAGG
oar-mir-221-p5	F: CCTGCTGGACCTGGCATACAAT	65	60
	R: ATCCAGTGCAGGGTCCGAGG
U6	F: ATCCAGTGCAGGGTCCGAGG	65	60
	R: TGAAGCGTGCTCGCTTCGGC

样本 Sample	原始数据 Raw data	有效数据 Valid data	参考基因组比对率 Mapping rate	Q20/%	Q30/%	GC含量/% GC content
Sad_1	94 092 386	91 107 390	86 158 306(94.57%)	99.96	97.78	45.00
Sad_2	97 790 686	94 803 474	89 533 252(94.44%)	99.96	97.71	45.00
Sad_3	89 825 602	86 701 034	80 875 819(93.28%)	99.97	98.16	48.50
C_1	69 188 934	67 121 848	62 676 263(93.38%)	99.84	97.85	46.00
C_2	75 845 456	73 401 010	67 841 183(92.43%)	99.84	97.91	45.50
C_3	77 539 444	75 024 956	69 565 183(92.72%)	99.82	97.81	46.00

样本 Sample	原始数据 Raw data	有效数据 Valid data	参考基因组比对率 Mapping rate	Q20/%	Q30/%	GC含量/% GC content
Sad_1	10 567 624	7 951 578	7 868 882 (98.96%)	97.92	96.91	51.40
Sad_2	9 796 916	6 971 109	6 893 729(98.89%)	97.92	96.96	51.22
Sad_3	10 036 758	6 868 427	6 794 248 (98.92%)	97.29	96.07	51.11
C_1	11 418 221	5 627 660	5 587 704 (99.18%)	99.29	97.37	51.24
C_2	9 754 842	5 237 938	5 189 225 (99.07%)	99.30	97.44	51.07
C_3	10 715 275	7 225 151	7 142 062(98.85%)	99.23	97.18	50.95

[1]	LUO Ruijie, WANG Jiankui, CAO Suying. Integrated Sequencing Analysis of lncRNA and mRNA Related to Ancestral-like Coarse in Aohan Fine Wool Sheep [J]. Acta Veterinaria et Zootechnica Sinica, 2025, 56(6): 2685-2700.
[2]	SHI Shanshan, WAN Qiongfei, XU Yingxin, WANG Qiushuo, ZHANG Linlin, GUO Yiwen, HU Debao, GUO Hong, DING Xiangbin, LI Xin. Sequencing and Bioinformatics Analysis of miRNAs at Different Developmental Stages of Bovine Skeletal Muscle [J]. Acta Veterinaria et Zootechnica Sinica, 2025, 56(6): 2701-2710.
[3]	ZHU Haiyan, ZHANG Jingyi, YAN Xueyong, LIANG Haiping, WEI Qing, CAO Ji, HUANG Jianzhen. Investigating the Molecular Mechanisms of Photoperiod 's Effect on Egg-Laying Performance of Taihe Black-Bone Silky Fowls Based on RNA-seq Analysis [J]. Acta Veterinaria et Zootechnica Sinica, 2025, 56(5): 2123-2135.
[4]	GUO Yanyan, ZHANG Yuxin, LU Rui, LI Yupeng, CHEN Longbin, ZHANG Jinlong, YAO Dawei, RUAN Weibin, ZHANG Xiaosheng, GUO Xiaofei. Research Progress on the Proliferation and Differentiation of Granulosa Cells at Various Follicular Development Stages in Mammal [J]. Acta Veterinaria et Zootechnica Sinica, 2025, 56(4): 1484-1493.
[5]	WANG Ying, ZHANG Jiaojiao, WANG Xianzhong, QUAN Fusheng. Advances in Autophagy of Ovarian Granulosa Cells [J]. Acta Veterinaria et Zootechnica Sinica, 2025, 56(4): 1508-1517.
[6]	YE Rungen, LIU Yuanbo, LU Lili, Collins Amponsah Asiamah, SU Ying*. Expression of miR-215-5p in Leizhou Black Duck Tissues and Its Effect on Follicular Granulosa Cells Proliferation and Apoptosis [J]. Acta Veterinaria et Zootechnica Sinica, 2025, 56(4): 1722-1730.
[7]	MIELIE·Madaniyati , SUN Meng, CHU Guiyan. The Regulatory Function of the Hedgehog Signaling Pathway in Follicle Development and Steroidogenesis of Animal Ovary [J]. Acta Veterinaria et Zootechnica Sinica, 2025, 56(3): 969-978.
[8]	CHEN Qiong, MAO Shuaixiang, WU Longfei, YANG Chuang, SUN Baoli. lncRNA Expression Characteristics in Semitendinosus Muscle of Leiqiong Cattle and Lufeng Cattle and Its ceRNA Network Analysis in Skeletal Muscle Development and Fat Deposition [J]. Acta Veterinaria et Zootechnica Sinica, 2025, 56(3): 1203-1215.
[9]	YANG Yang, LI Liangyuan, WAN Pengcheng, LU Shouliang, LIU Changbin, YANG Hua, WANG Limin, DAI Rong, ZHOU Ping. Screening and Analysis of Core Genes and Key lncRNAs for Seasonal Estrus Traits in Sheep [J]. Acta Veterinaria et Zootechnica Sinica, 2025, 56(3): 1264-1277.
[10]	LU Jian, MA Meng, GUO Jun, WANG Xingguo, DOU Taocun, HU Yuping, WANG Qiang, LI Yongfeng, SHAO Dan, TONG Haibing, GUO Jie, QU Liang. Studies on Key Genes and Signaling Pathways of Regulation of Energy Restriction during Rearing and Conversion to Ad libitum on the Reproductive Organ Development of Hens at the Initiation of Laying Period [J]. Acta Veterinaria et Zootechnica Sinica, 2025, 56(2): 737-754.
[11]	WANG Lei, BAI Shaocheng, WANG Sen, BAO Zhiyuan, CAI Jiawei, LIU Yan, ZHAO Bohao, WU Xinsheng, CHEN Yang. Effect of SRD5A2 on the Expression of Genes Related to Proliferation, Apoptosis and Steroid Hormone Synthesis in Rabbit Granulosa Cells [J]. Acta Veterinaria et Zootechnica Sinica, 2025, 56(1): 259-268.
[12]	Yuxin GAO, Qing LIU, Jilan CHEN, Hui MA. Research Advances in the Mechanism of Parasite-host Interaction Mediated by miRNAs [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(9): 3812-3823.
[13]	Yaxuan MENG, Yan LIU, Jing WANG, Guoshun CHEN, Tao FENG. Research Progress in the Effect of Oxidative Stress on Ovarian Function in Female Livestock [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(7): 2825-2835.
[14]	Jingyu LI, Jinming CHEN, Mingyi ZHANG, Shanshan ZHAO, Deliang TAO, Junke SONG, Xin YANG, Yingying FAN, Guanghui ZHAO. Identification and Analysis of miRNAs of Neospora caninum [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(7): 3085-3093.
[15]	Yan ZHANG, Meijin WU, Jiahao ZHOU, Hongxiu DIAO. The Effect of Doxorubicin Treatment on the Differential Expression of lncRNAs in Canine Mammary Tumor CHMp Cell Line [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(6): 2716-2726.

Construction of Ovarian ceRNA Networks and Screening of Key miRNA in Two Different Breeds of Sheep Based on Whole Transcription Sequencing

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 11

References 45

Related Articles 15

Recommended Articles

Metrics

Comments