山羊痘病毒锚蛋白ORF140对NF-κB信号通路的调控作用

doi:10.11843/j.issn.0366-6964.2024.11.034

Abstract

Abstract:

The NF-κB pathway is an important target for regulation of host immune function by viruses and plays an important role in the pathogenesis of viral infections. It has shown that the ankyrin protein encoded by some poxviruses regulates the NF-κB pathway in a variety of ways, but how the ankyrin protein encoded by goat poxvirus (GTPV) regulates the NF-κB pathway remains to be explored. In this paper, we aim to investigate the effects of the ankyrin protein ORF140 encoded by GTPV on the NF-κB pathway. The effects of ankyrin protein ORF140 on the NF-κB pathway were detected using dual luciferase reporter system, its localization in HEK293T cells was observed by indirect immunofluorescence and its effects on expression of the factors associated with the activated NF-κB pathway at different time points by Western blot. Results showed that ORF140 was able to inhibit the NF-κB pathway. It was localized in the cytoplasm and did not undergo nuclear translocation even when the NF-κB pathway was activated. ORF140 was able to inhibit the nuclear translocation of NF-κB-p65 and the degradation of p-IκBα. GTPV ORF140 localizes in the cytoplasm and downregulates NF-κB transcriptional activity by inhibiting the nuclear translocation of NF-κB-p65 and the degradation of p-IκBα.

Key words: goat poxvirus, ankyrin protein, NF-κB, ORF140

CLC Number:

S852.659.1

Qiuyuan FENG, Hanwei YU, Yunfeng YANG, Hui ZHANG, Weiyi HANWU, Xintong SUN, Xiaolong SHAO, Junlin LIU, Guohua CHEN, Zhizhong JING, Yixia CHEN. Regulation of the NF-κB Signaling Pathway by Goat Poxvirus Ankyin Protein ORF140[J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(11): 5191-5199.

Figures/Tables 5

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Regulation of the NF-κB Signaling Pathway by Goat Poxvirus Ankyin Protein ORF140

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