热带无爪螨主要变应原Blo t 5和Blo t 21融合表达质粒诱导小鼠产生IgG和调节性T细胞

doi:10.11843/j.issn.0366-6964.2022.04.022

Abstract

Abstract: To develop DNA vaccine against Blomia tropicalis allergy, we constructed eukaryotic expression vector pVAX1-PADRE-Blo t 5-21-CpG of major allergen fusion gene of Blomia tropicalis by codon optimization of Blo t 5 and Blo t 21 gene sequences. To verify the immune effect of the eukaryotic expression vector, BALB/c mice were immunized with 100 μg/100 μL of pVAX1-PADRE-Blo t 5-21-CpG by subcutaneous injection (n=6)，and the serum levels of specific IgG, IgG1 and IgG2a were detected by ELISA. The changes of regulatory T cells in spleen lymphocytes of mice were detected by flow cytometry. The results showed that the eukaryotic expression plasmid pVAX1-PADRE- Blo t 5-21-CpG could induce the increase of specific IgG and IgG2a levels in mice, and the increase of IgG2a/IgG1 ratio suggested a bias towards a Th1 type immune response in mice after immunization. In addition, the plasmid pVAX1-PADRE-Blo t 5-21-CpG can induce higher levels of CD4⁺CD25⁺Foxp3⁺ T cells in mice, suggesting that the eukaryotic expression plasmid may induce immunosuppression.

Key words: Blomia tropicalis, allergen, eukaryotic expression vector, DNA vaccine

CLC Number:

S852.746

LUO Ying, PENG Meiqi, XIAO Zhengpan, LUO Chenghui, WANG Dayong, PEI Yechun. Expression of Blomia tropicalis Major Allergens Blo t 5 and Blo t 21 Induced the Production of IgG and Regulatory T Cells in Mice[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(4): 1220-1230.

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Expression of Blomia tropicalis Major Allergens Blo t 5 and Blo t 21 Induced the Production of IgG and Regulatory T Cells in Mice

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