脱氧雪腐烯醇(DON)对牛卵母细胞体外成熟及发育能力的影响

doi:10.11843/j.issn.0366-6964.2021.09.010

Abstract

Abstract: This study aimed to investigate the effect and mechanism of deoxynivalenol (DON) on the maturation and development of bovine oocytes in vitro. Bovine oocytes were matured in vitro in the mature solution containing different concentrations of DON (0,50,250,500,1 000 ng·mL^-1), respectively. The expansion degree of cumulus cell and first polar body discharge rate were detected to construct DON toxicity model. Mitochondrial distribution of oocytes, fertile cleavage rate, blastocyst development rate were determined to evaluate the effect of DON on bovine cocytes in vitro maturation and development. And the oxidative stress,where the level of the ROS and GSH and the mRNA expression of CAT、GPx4 were detected to reveal the mechanism of DON action. It was found that the first polar body excretion of the oocytes was reduced by 250 and 500 ng·mL^-1 (P<0.05) and 1 000 ng·mL^-1 of DON(P<0.01).The expansion degree of cumulus cells was significant inhibited by 250 ng·mL^-1 of DON (P<0.05), extremely significant inhibited by 500 and 1 000 ng·mL^-1 of DON (P<0.01). In subsequent experiments, 500 ng·mL^-1 of DON was selected as the toxicity model (DON group) to compare with the group without DON (control group). The results showed that the mitochondrial uniform distribution was significanly different between the control group and DON group (60.2% vs. 40.0%, P<0.05). While fertile cleavage rate ((33.6±3.6)% vs. (67.7±2.6)%, P<0.05) and blastocyst rate ((0.0±0.0)% vs.(18.3±2.2)%, P<0.05) were significantly reduced in DON group compared with the control group. The relative level of ROS in the DON group was higher than that in the control group (1.6 vs.1.0, P<0.05), however, the relative level of GSH in the DON group was lower than that in the control group (0.4 vs.1.0, P<0.05), and the relative mRNA expression level of antioxidant related genes CAT and GPX4 in the DON group were lower than those in the control group (0.3 vs.1.0; 0.6 vs. 1.0, P<0.05). The results indicates that DON inhibits the maturation in vitro and early embryo development of bovine oocyte, and the mechanism is related to DON disturbing the balance of antioxidant system of bovine oocytes.

Key words: deoxynivalenol, bovine, oocytes, in vitro maturation

CLC Number:

SUN Huan, SUN Kejia, JIANG Xiaolong, LIU Aiju, MA Xiaofei, HAN Hongye, YAO Dawei, MA Yi, TIAN Shujun. Effect of Deoxynivalenol(DON) on Maturation and Development of Bovine Oocytes in vitro[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(9): 2475-2483.

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Effect of Deoxynivalenol(DON) on Maturation and Development of Bovine Oocytes in vitro

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