Abstract: In November 2020, the imported cattle in Binzhou and Dongying of Shandong Province showed circumscribed skin nodules, skin wounds and scab, which were suspected of lumpy skin disease (LSD). In order to confirm the cause of the disease in the two places and understand its genetic evolution relationship, fluorescent quantitative PCR(qPCR) was used for etiological diagnosis. GCPR gene was amplified by PCR and the alignment analysis and phylogenetic analysis were carried out. Lumpy skin disease virus (LSDV) was detected with qPCR in the collected samples. The disease occurred in the two cities were finally confirmed as LSD. The alignment analysis showed that there were 12 nucleotides insertion in the GCPR gene of China/SDBinzhou/2020 and China/SDDongying/2020, which was same as the insertion sequence in GCPR gene of vaccine strains, Neethling vaccine LW 1959 and Neethling-LSD vaccine-OBP, and vaccine-like strain, Saratov. Phylogenetic analysis showed that GCPR of China/SDBinzhou/2020 and China/SDDongying/2020 were closely related to LSDV strains found in Xinjiang. At the same time, LSDV strain found in China were allocated in a large branch. In conclusion, the disease occurred in Binzhou and Dongying were confirmed as LSD, which was the first confirmation of the imported case of LSD in Shandong province.

Key words: lumpy skin disease, lumpy skin disease, fluorescent quantitative PCR, exotic animal diseases