静脉滴注心房利钠肽对阿勒泰羊血液脂代谢激素的影响及尾脂转录组分析

doi:10.11843/j.issn.0366-6964.2021.011.009

Abstract

Abstract: The aim of this study was to investigate the effects of intravenous infusion of atrial natriuretic peptide (ANP) on blood lipid metabolism hormones and tail lipid transcriptome of sheep, and to provide reference for elucidating the mechanism of ANP in regulating lipid metabolism in sheep. Eight healthy Altay ewes with the age of 1.5-year-old, body weight (BW) of (45.6±6.5)kg were selected, and self control design was used. During the control period, 100 mL saline was infused intravenously in 45 min, and during the trial period, 100 mL of ANP saline solution with a dose of 1.125 μg·kg^-1BW was infused intravenously in 45 min. Intravenous infusion lasted for 4 days. At the end of each period (4th day), blood samples were collected at 0, 15, 30, 45, 60, 90 and 120 min after infusion, and the content of ANP, adiponectin, insulin, leptin and cyclic guanosine (cGMP) in plasma were deterimed. The sheep tail fat samples were collected for trans-criptome sequencing and biological information analysis, and qRT-PCR was used to detect the change of relative candidate genes expression. The results showed that: 1) After intravenous infusion of ANP, compared to the control period, the plasma ANP level at 30 and 90 min was significantly higher during the trial period(P<0.05); Adiponectin level at 30 min significantly increased (P<0.05), and at 90 min extremely significantly increased (P<0.01); The plasma cGMP level at 30 and 90 min extremely significantly increased(P<0.01), and at 45 and 60 min significantly increased (P<0.05); The plasma insulin at 0, 30 and 120 min extremely significantly increased(P<0.01), at 15, 45, 60 and 90 min significantly increased (P<0.05); The plasma leptin level at 0 and 15 min significantly increased(P<0.05), at 30 min extremely significantly increased(P<0.01). 2) Transcriptome analysis showed that there were 3 686 differentially expressed genes, of which 1 482 genes were up-regulated and 2 204 genes were down-regulated; GO function enrichment analysis showed that differentially expressed genes were significantly enriched into 118 biological functions, mainly related to mitochondrial respiratory chain and oxidative phosphorylation; KEGG pathways analysis revealed that differentially expressed genes were significantly enriched into 46 pathways, mainly involved in ribosome, thermogenesis, oxidative phosphorylation and regulating lipolysis in adipocytes and other signaling pathways; qRT-PCR analysis results showed that the expression trends of AQP7, FABP4, PLIN5, ADIPOR2, MGLL,IDE and ACSL1 were consistent with the RNA-seq results, which proved that the trans-criptome sequencing data was reliable. In conclusion, exogenous ANP promotes lipolysis by changing the level of lipid metabolism-related hormones and increasing the oxidative phosphorylation and thermogenesis of adipose tissue in sheep.

Key words: atrial natriuretic peptide, fat metabolism, RNA-seq technology, tail fat, lipolysis

CLC Number:

S826.2

MA Xin, YANG Guang, LI Xiangyu, LIU Yueyue, CHEN Yong. Effects of Intravenous Infusion of Atrial Natriuretic Peptide on Blood Lipid Metabolism Hormones and Transcriptome Analysis of Tail Adipose Tissue of Altay Sheep[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(11): 3076-3088.

References 35

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Effects of Intravenous Infusion of Atrial Natriuretic Peptide on Blood Lipid Metabolism Hormones and Transcriptome Analysis of Tail Adipose Tissue of Altay Sheep

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