BMPR-IB基因在寒泊羊群体中的增羔效应、遗传规律及育种应用分析

doi:10.11843/j.issn.0366-6964.2020.04.005

Abstract

Abstract: The aims of this study were to reveal the polymorphism and genetic regularity of the BMPR-IB gene in Hanper sheep population, and to explore the scientificity to use the A → G mutation (FecB mutation) at the 746th base of the BMPR-IB gene as a molecular marker for breeding sheep prolific breed. In this study, a total of 1 267 sheep individuals were selected, blood samples were collected from healthy breeding rams, breeding ewes, and lambs of the Hanper sheep breeding core population. PCR-RFLP method was used to determine the genotypes of BMPR-IB gene locus in sheep population and analyze the population genetics parameters. The statistics of the lambing records of a total of 980 breeding ewes were analyzed, and the effects of FecB mutation, parity and lambing season on litter size were analyzed. Genotype frequency of 167 healthy lambs from the offspring of the designed hybrid combination was counted. The results showed that BMPR-IB gene had BB, B+ and ++ genotypes in Hanper sheep population, the frequencies of the 3 genotypes were 1.97%, 73.40% and 24.63%, respectively, the allele frequencies of B and + were 38.67% and 61.33%, respectively. The litter size of breeding ewes with the BB, B+ and ++ genotypes were 2.69, 1.91 and 1.57, respectively, the average litter size of the current Hanper sheep population was 1.85; One copy of B allele was added to the individual of Hanper sheep by breeding, the litter size was expected to increase by 0.44. The ratio of B+ and ++ genotypes in the offspring of hybrid combination of B+×++ was 1.11:1, and the ratio of BB and B+ genotypes in the offspring of hybrid combination of B+×BB was 0.82:1, both conforming to Mendelian separation law. It was predicted that after 6 generations of breeding, litter size of Hanper sheep ewes could basically achieved 2 lamds. The results of this study provide a theoretical basis for the development of breeding and selection schemes in sheep breeding practice.

Key words: BMPR-IB, Hanper sheep, litter size, heredity rule

CLC Number:

S826.2

MA Xiaofei, LIU Aiju, HAN Hongye, SUN Huan, SUN Kejia, LI Xiaoshuang, SUN Shuchun, TIAN Shujun. Analysis of BMPR-IB Gene's Effect on Litter Size, Its Heredity Rule and Breeding Application in Hanper Sheep[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(4): 688-700.

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Analysis of BMPR-IB Gene's Effect on Litter Size, Its Heredity Rule and Breeding Application in Hanper Sheep

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