H5、H7和H9亚型禽流感病毒四重荧光RT-PCR检测方法的建立与应用

doi:10.11843/j.issn.0366-6964.2020.026

Abstract

Abstract: To shorten the detection time, a quadruple fluorescence RT-PCR method was established for detecting avian influenza virus with the related probes and primers designed and synthesized by analyzing the conservative sequences of M gene, HA gene of H5 subtype, H7 subtype and H9 subtype. This method can detect avian influenza virus, as well as H5 subtype, H7 subtype, and H9 subtype simultaneously. The results showed that the method was quick, specific, and the detection limitation was 10^-5 ng·μL^-1. Sixty avian influenza viruses were detected from 384 chicken swab samples from 13 living birds markets with the method. All the detected avian influenza viruses were H9 subtype. The results were consistent with the method reported in standard, NY/T 772-2013. The κ value for these two methods was 1 (P<0.001). This method can realize the detection of avian influenza virus which has the characteristics of safety, specificity, rapidity, sensitivity, simplicity and high throughput. It will play an important role in the rapid detection of avian influenza virus.

Key words: avian influenza virus, H5 subtype, H7 subtype, H9 subtype, quadruple real-time fluorescence RT-PCR

CLC Number:

S852.659.5

WANG Suchun, ZHONG Huanxiang, JIANG Nan, JIANG Lijian, PAN Zihao, SUN Fuliang, LIU Hualei, HUANG Baoxu, WANG Kaicheng. Establishment of the Quadruple Real-time Fluorescence RT-PCR for Detection of H5, H7 and H9 Subtypes Avian Influenza Virus[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(6): 1429-1437.

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Establishment of the Quadruple Real-time Fluorescence RT-PCR for Detection of H5, H7 and H9 Subtypes Avian Influenza Virus

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