PRRSV VR2332弱毒株经滴鼻、注射途径接种仔猪的病毒血症和抗体产生的差异分析

doi:10.11843/j.issn.0366-6964.2019.02.015

Abstract

Abstract:

The study was conducted to understand the difference of viremia, anti-PRRSV antibody and anti-PRRSV neutralizing antibody in piglets vaccinated against porcine reproductive and respiratory syndrome virus (PRRSV) VR2332 attenuated strain which was given by nasal drip and injection. Nine 20-day-old health piglets were randomly selected and separated into 3 groups which designated as nose dropping group, injection group and control group respectively. Blood samples were collected by the anterior vena cava at day 0, 3, 7, 11, 15, 19, 23, 27, 31, 35, 39, 43 after infection, spleen, submandibular lymph nodes, inguinal lymph nodes and mesenteric lymph nodes were obtained at day 43, PAMs were collected in a sterile room meanwhile. Viral load in piglets was detected with the real-time fluorescent quantitative PCR. The anti-PRRSV antibody titer was tested by ELISA, and the anti-PRRSV neutralizing antibody titer was detected by the method based on PAMs. The results showed that, ①PRRSV started to replicate in only 2 piglet's sera at day 3 after infection, viremia peaked at day 15-19, the virus copy number was greater than 10⁴ copies·μL^-1 and viremia disappeared after day 27 in nose dropping group. PRRSV started to replicate in all 3 piglet's serum at day 3 after infection, viremia peaked at day 15-23, the virus copy number was up to 10⁵ copies·μL^-1 at day 19 and viremia disappeared after day 31 in injection group. ②PRRRSV can be detected in these lymph nodes and PAMs, and the virus copy number of injection group was greater than nose dropping group. ③Anti-PRRSV antibody was produced in piglets at day 27 after infection from serum, and anti-PRRSV antibody levels of injection group was always higher than nose dropping group. ④Anti-PRRSV neutralizing antibody titers was 1:2 at day 43 and lower than 1:2 before day 35 in nose dropping group. Anti-PRRSV neutralizing antibody titers was 1:4 at day 27, 1:8 at day 35, 1:4 at day 43 and lower than 1:2 at day 3, 11 and 19 in injection group. The results showed that the virus copy number in injection group was always ten to one hundred times higher than that of nose dropping group, and duration of viremia was longer. Anti-PRRSV antibody producing time in injection group was earlier than nose dropping group, and antibody levels was higher. Anti-PRRSV neutralizing antibody levels in nose dropping group was very low, anti-PRRSV neutralizing antibody producing time in injection group was earlier than nose dropping group, and antibody levels were higher. In conclusion, higher level neutralizing antibody can be stimulated in piglets after inoculating PRRSV VR2332 attenuated strain by injection than by nasal drip, but higher level viremia also can be induced meanwhile.

CLC Number:

S858.285.3

LI Wen, MA Sixu, LI Xiangtong, SUN Yangyang, WEI Fengling, XU Ruiqin, YANG Guoyu, XIA Ping'an, ZHANG Gaiping. The Variance Analysis of Viremia and Antibody in Piglets Vaccinated against PRRSV VR2332 Attenuated Strain via Nasal Drip and Injection[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(2): 373-381.

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The Variance Analysis of Viremia and Antibody in Piglets Vaccinated against PRRSV VR2332 Attenuated Strain via Nasal Drip and Injection

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