表没食子儿茶素没食子酸酯对牛冷冻精液品质及受精能力的影响

doi:10.11843/j.issn.0366-6964.2018.10.011

Abstract

Abstract:

The present study aimed to investigate the effects of epigallocatechin-3-gallate (EGCG) on bovine frozen-thawed semen quality and subsequent fertilization ability. The frozen-thawed semen were incubated with different concentrations of EGCG(0, 5, 10 and 20 μmol·L^-1) at 38.5℃ for 1 h. Kinetic parameters, structural integrity, activities of related enzymes and subsequent fertilization ability were measured. The results revealed that supplementation with 10 μmol·L^-1 EGCG significantly improved the percentage of total motile sperms(TM,%) and the beat cross frequency (BCF, Hz, P<0.05). The percentages of spermatozoa with intact plasma membrane and acrosome were significantly higher in 5, 10 and 20 μmol·L^-1 EGCG treated groups compared with the control group(P<0.05). 5 and 10 μmol·L^-1 EGCG led to higher mitochondrial membrane integrity(P<0.05). The activities of SOD and CAT in spermatozoa treated with 10 and 20 μmol·L^-1 EGCG were significantly increased, while the LDH activity and MDA level were markedly decreased in 10 μmol·L^-1 EGCG group(P<0.05). The cleavage and blastocyst development rates of in vitro-produced bovine embryos originating from 10 and 20 μmol·L^-1 EGCG-treated spermatozoa were significantly higher than for embryos from other groups(P<0.05). In summary, 10 μmol·L^-1 EGCG can enhance the quality and subsequent fertilization ability of bovine frozen-thawed semen.

CLC Number:

S823.2

HUANG Zi-qiang, WANG Ling-ling, PANG Yun-wei, HAO Hai-sheng, DU Wei-hua, ZHAO Xue-ming, LIU Yan, ZHU Hua-bin. Effects of Epigallocatechin-3-gallate on Bovine Frozen Semen Quality and Subsequent Fertilization Ability[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(10): 2154-2162.

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Effects of Epigallocatechin-3-gallate on Bovine Frozen Semen Quality and Subsequent Fertilization Ability

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