猪基因共表达网络模块的构建及功能分析

doi:10.11843/j.issn.0366-6964.2017.11.023

Abstract

Abstract:

The present study aimed to construct gene co-expression network modules in pig, and explore functional genes. The 24 modules were constructed using RNA-seq dataset by Weighted Gene Co-expression Network Analysis, which were enriched in biological process, KEGG pathway, disease and tissue specificity. Modules showed significant functional specificity, and were correlated with specific development stages and metabolic pathways of organs. In addition, signaling pathways within the same module had regulation relationships with each other. Furthermore, modules enriched genes in diseases related to metabolic, nervous system and cancer, and also modules were located at the specific tissues. Moreover, calcium channel, PI3K-Akt signaling pathway, MAPK signaling pathway and ubiquitin mediating signaling pathway played key roles in different modules. The genes related to oxytocin and circadian entrainment pathways performed important regulatory functions in reproduction. SIX1 and PRKAG3 involved in development of muscles. Finally, the construction and analysis of gene co-expression regulation network can provide new ideas and methods to discover specific gene resource in Chinese local pig breeds and enrich the theories of molecular breeding.

CLC Number:

S828.2

WEI Kai, ZHANG Ting-ting, MA Lei. Construction and Functional Analysis of Gene Co-expression Network Modules[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(11): 2205-2215.

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Construction and Functional Analysis of Gene Co-expression Network Modules

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