绒山羊附睾上皮细胞培养方法的建立及其生长特性

doi:10.11843/j.issn.0366-6964.2017.07.005

Abstract

Abstract:

The aim of this study was to establish a simple and feasible in vitro culture system for high purity epididymal epithelial cells from cashmere goat. The primary cells were obtained from epididymal caput of 10-12 months cashmere goat using enzymatic dispersion and improved tissue-piece digestion method. The cells were identified by immunocytochemistry and immunofluorescence methods. Cell purity and proliferation were also investigated respectively by flow cytometry and CCK method. Ultrastructures of epididymal epithelial cells were observed under transmission electron microscope. The results showed that the cells obtained by both methods were in island-like clonal growth and good proliferation ability, and possessed active secretory and metabolic function. The expression of epithelial special cytokeratin 18 and epididymal special glutathione peroxidase 5(GPX5) and the result of flow cytometry showed that both methods could obtain high purity epididymal epithelial cells. The primary cells obtained by improved tissue-piece digestion method took a long culture time, compared to that the primary cells obtained by enzymatic dispersion method which took a short culture time with more cells. These results provided a good foundation for further study on the function of epididymal epithelial cells of cashmere goat.

CLC Number:

S827
Q24

FAN Xiao-mei, ZHANG Tong, LI Rui-lan, BIAN Xiao-na, ZHANG Jia-xin. Establishment of Culture Method and Growth Characteristics for Epididymal Epithelial Cells from Cashmere Goats[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(7): 1212-1220.

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References

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Establishment of Culture Method and Growth Characteristics for Epididymal Epithelial Cells from Cashmere Goats

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