小型猪复合麻醉剂对大鼠中枢神经系统p-p38MAPK蛋白表达的影响

doi:10.11843/j.issn.0366-6964.2015.07.024

Abstract

Abstract:

In order to investigate the expression of p-p38 in central nervous system in rats anesthetized with the Combined Anaesthetic for Miniature Pigs (XFM)，30 rats were divided randomly into two groups：control group and XFM group.XFM group consisted of four subgroups：M1 subgroup (rats received XFM intraperitoneally and then waiting the disappearance of right reflection)；M2 subgroup (rats received XFM intraperitoneally and then waiting for 1 h after the disappearance of right reflection)；M3 subgroup (rats received XFM intraperitoneally and then waiting the recovery of right reflection) and M4 subgroup (rats received XFM intraperitoneally and then waiting for 1 h after the recovery of right reflection).The brain tissues were taken when reaching each time point.With the method of RT-PCR，expression of p38 mRNA were detected，and the expression of p-p38 in central nervous system was detected with Western blot.The results showed that，compared with the control group，the transcription of p38 mRNA on cerebellum and thalamus in experiment groups were significant lower than that in control group (P<0.05)，and p38 mRNA increased at group M3 and group M4，but significantly lower than control group (P<0.05).In group M1 and group M2，the expression of p-p38 in cerebellum and thalamus were significantly lower than that in the control group (P<0.05)，but the expression of p-p38 in cerebellum，hippocampus，brainstem increased significantly after administration of XFM (P<0.05) in group M1，group M2，and group M3，compared with the control group，and in group M4，the expression of p-p38 decreased，were no difference with the control group (P>0.05).These results indicated that the decrease of the expression of p-p38 in cerebellum and thalamus were induced by XFM，and these changes of p38 mRNA and p-p38 maybe one of the anaesthetic mechanisms of XFM.

CLC Number:

S859.791

LI Xin，JIANG Sheng，HOU Jin-long，FAN Hong-gang，WANG Hong-bin. Effects of the Combined Anaesthetic for Miniature Pigs on the Expression of p-p38MAPK in Central Nervous System of Rats[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(7): 1253-1258.

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References

［1］王洪斌，范宏刚，卢德章，等．噻环乙胺对大鼠不同脑区NOS活性及NO产量和cGMP含量的影响［J］．畜牧兽医学报，2008，39（11）：1599-1605．
WANG H B，FAN H G，LU D Z，et al.Effects of tiletamine on the activity of NOS，NO production，and cGMP content in rat different brain regions［J］.Acta Veterinaria et Zootechnica Sinica， 2008，39(11)：1599-1605.（in Chinese）
［2］范宏刚，卢德章，胡魁，等．小型猪复合麻醉剂对小型猪血流动力学及血浆中肾素-血管紧张素-醛固酮系统的影响［J］．畜牧兽医学报，2009，40（8）：1244-1248．
FAN H G，LU D Z，HU K，et al.Effects of the combined anaesthetic for miniature pigs on hemodynamics and renin-angiotensin-aldosterone-system in miniature pigs［J］.Acta Veterinaria et Zootechnica Sinica，2009，40(8)：1244-1248.（in Chinese）
［3］张丽华，侯蕾娜，邓立琴，等．脊髓背角 p38MAPK 活化在瑞芬太尼诱发术后痛觉过敏中的作用［J］．中国疼痛医学杂志，2014，20（9）：620-624．
ZHANG L H，HOU L N，DENG L Q，et al.The role of p38MAPK activation in spinal cord dorsal horn in reminfentanil induced postoperative hyperalgesia［J］.Chinese Journal of Pain Medicine，2014，20 (9)：620-624.（in Chinese）
［4］王强，由振东，石雪银，等．异氟醚吸入对新生大鼠认知功能的影响及p38MAPK抑制剂的干预作用［J］．山东医药，2011，51（19）：21-23．
WANG Q，YOU Z D，SHI X Y，et al.Effects of isoflurane on the cognitive function of neonatal rats and intervention roles of p38MAPK inhibitor［J］.Shandong Medical Journal，2011，51(19)：21-23.（in Chinese）
［5］LYCNCH M A.Long-term potentiation and memory［J］.Physiol Rev，2004，84(1)：87-136.
［6］李玉娟，柳垂亮，张静，等．异氟醚和七氟醚对新生大鼠皮质凋亡以及JNK和p38表达的不同影响［J］．中国病理生理杂志，2011，27（1）：72-76．
LI Y J，LIU C L，ZHANG J，et al.Effects of isoflurane and sevoflurane on apoptosis of cortical neuron in neonatal rats and the role of MAPKs pathway［J］.Chinese Journal of Pathophysiology，2011，27(1)：72-76.（in Chinese）
［7］曹慧灵，但伶，邓必高．异丙酚抑制脂多糖致大鼠脑损伤时p38MAPK的活化［J］．中国病理生理杂志，2011，27（8）：1639-1642．
CAO H L，DAN L，DENG B G.Propofol inhibits activation of p38 MAPK in rat brain tissues with LPS-induced brain injury［J］.Chinese Journal of Pathophysiology，2011，27(8)：1639-1642.（in Chinese）
［8］潘彩飞，祝胜美．异丙酚通过抑制p38激活下调氨处理的大鼠脑星型胶质细胞AQP4的表达并减轻细胞水肿［J］．中国病理生理杂志，2010，26（1）：96-100．
PAN C F，ZHU S M.Propofol attenuates aquaporin-4 over-expression through p38 pathway on ammonia-induced neocortical astrocyte in rats［J］.Chinese Journal of Pathophysiology，2010，26(1)：96-100.（in Chinese）
［9］JI R R，KAWASAKI Y，ZHUANG Z Y，et al.Protein kinases as potential targets for the treatment of pathological pain［J］.Handb Exp Pharmacol，2007（177）：359-389.
［10］COOGAN A N，O’NEILL L A，O’CONNOR J J.The P38 mitogen-activated protein kinase inhibitor SB203580 antagonizes the inhibitory effects of interleukin-1β on long-term potentiation in the rat dentate gyrus in vitro［J］.Neuroscience，1999，93(1)：57-69.
［11］王飞，李玉娟，曾敏婷，等．右美托咪啶通过抑制 p38 和 JNK 活化减少异氟醚诱导的新生大鼠海马神经元凋亡［J］．中国病理生理杂志，2013，29（9）：1651-1656．
WANG F，LI Y J，ZENG M T，et al.Dexmedetomidine reduced isoflurane- induced neuroapoptosis by inhibiting activation of p38 and JNK proteins in hippocampus of neonatal rats［J］.Chinese Journal of Pathophysiology，2013，29(9)：1651-1656.（in Chinese）
［12］刘永哲，高明龙，张宏，等．异氟醚和七氟醚对脑室内注射β-淀粉样蛋白单体的老年大鼠海马内磷酸化JNK p38及tau蛋白的影响［J］．中国药物与临床，2009，9（7）：553-556．
LIU Y Z，GAO M L，ZHANG H，et al.Effect of isoflurane and sevoflurane on hippocampal expressions of p-JNK，p-p38 and p-tau in aged rats after intracerebroventricular injection of amyloid-beta protein［J］.Chinese Remedies & Clinics，2009，9(7)：553-556.（in Chinese）

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Effects of the Combined Anaesthetic for Miniature Pigs on the Expression of p-p38MAPK in Central Nervous System of Rats

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