miR-21靶向猪TSC1和PPP3CA基因的初步鉴定

doi:10.11843/j.issn.0366-6964.2013.06.023

Abstract

Abstract:

The study was to investigate the regulation mechanism of miR-21 in skeletal muscle development. Using computational target prediction programs, we identified TSC1 and PPP3CA genes as the potential targets of miR-21 which can specify bind to their 3′ UTR regions. The 3′UTR regions of these two genes were amplified by PCR from Tongcheng pigs genomic DNA. The PCR productions were digested by double digestion with Not I and Xho I. We got the pCHE-TSC1-3′UTR and pCHE-PPP3CA-3′UTR vectors after digested products were ligated into the vector psiCHECK with the dual-luciferase gene. These vectors were transfected into porcine iliac endothelial cell line (PIEC) and porcine kidney epithelial cell line (PK15), and then the luciferase activity was tested by a dual-luciferase reporter assay. The results showed that the luciferase activity of experimental group was significantly lower than that of the control（P<0.05）both in PIEC and PK15. This study demonstrated that TSC1 and PPP3CA were the possible target genes of miR-21 and provides basic for further research the function of miR-21.

CLC Number:

S828.2

PENG Xing, XIE Bing-kun, TANG Zhong-lin, ZHOU Rong, AO Hong, HUANG Sheng-qiang, LI Kui. Preliminary Identification of miR-21 Targeted TSC1 and PPP3CA Genes of Pigs[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2013, 44(6): 985-992.

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Preliminary Identification of miR-21 Targeted TSC1 and PPP3CA Genes of Pigs

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