一株猪伪狂犬病病毒Bartha-K61疫苗变异株的分离鉴定及生物学特性分析

doi:10.11843/j.issn.0366-6964.2025.12.031

畜牧兽医学报 ›› 2025, Vol. 56 ›› Issue (12): 6293-6298.doi: 10.11843/j.issn.0366-6964.2025.12.031

一株猪伪狂犬病病毒Bartha-K61疫苗变异株的分离鉴定及生物学特性分析

胡文佳(), 时建皓(), 李佳妮, 殷雨晨, 张新月, 董海冰, 杨海燕*(), 张传美*()

青岛农业大学动物医学院, 青岛 266109

收稿日期:2024-08-28 出版日期:2025-12-23 发布日期:2025-12-24
通讯作者: 杨海燕,张传美 E-mail:2236091142@qq.com;2412373957@qq.com;hy386@126.com;zhangchuanmei100@163.com
作者简介:胡文佳(2001-)，女，山东泰安人，硕士生，主要从事预防兽医学研究，E-mail: 2236091142@qq.com
时建皓(1998-)，男，江苏宿迁人，硕士生，主要从事预防兽医学研究，E-mail: 2412373957@qq.com
第一联系人：
胡文佳与时建皓为同等贡献作者
基金资助:
2022年生猪病原及抗体的检测(横20220016)

Isolation, Identification and Biological Characterization of a Porcine Pseudorabies Virus Bartha-K61 Vaccine Variant

HU Wenjia(), SHI Jianhao(), LI Jiani, YIN Yuchen, ZHANG Xinyue, DONG Haibing, YANG Haiyan*(), ZHANG Chuanmei*()

College of Veterinary Medicine, Qingdao Agricultural University, Qingdao 266109, China

Received:2024-08-28 Online:2025-12-23 Published:2025-12-24
Contact: YANG Haiyan, ZHANG Chuanmei E-mail:2236091142@qq.com;2412373957@qq.com;hy386@126.com;zhangchuanmei100@163.com

摘要/Abstract

摘要：

为研究猪伪狂犬病病毒(pseudorabies virus，PRV)地方分离株的变异情况，本试验从烟台某猪场送检的病料中成功分离到一株PRV毒株，命名为JH2403。对其gB、gC、gD、TK基因进行遗传进化分析并研究其对小鼠及仔猪的致病性。结果显示：系统发育分析表明：该毒株gB、gC、gD、TK基因与疫苗株Bartha-K61相似性为99.9%~100%，3个位点AA发生突变。1×10³TCID₅₀·mL^-1病毒液0.2 mL·只^-1感染小鼠，第6天死亡率100%，小鼠脑膜充血且HE染色可见淋巴细胞浸润。仔猪致病性试验表明，高、低剂量攻毒组仔猪均出现发热、瘙痒等明显临床症状，但无死亡。综上，本研究成功分离到一株PRV毒株，与疫苗株Bartha-K61相似性最高，对小鼠的致病性较强，对仔猪致病性较低。本研究为山东省PRV遗传进化及流行病学研究提供一定参考。

关键词: 伪狂犬病病毒, 分离鉴定, 遗传变异, 致病性

Abstract:

To study the variation of porcine pseudorabies virus (PRV) local isolates, a PRV virus strain named JH2403 was successfully isolated from diseased materials sent to a pig farm in Yantai City. Perform genetic evolution analysis on its gB, gC, gD, TK genes and study of the pathogenicity of the isolates in mice and piglets. Phylogenetic analysis showed that the gB, gC, gD, TK genes of the strain had 99.9% to 100% homology with the vaccine strain Bartha-K61, with a total of 3 amino acid mutations. The pathogenicity test of mice showed that 1×10³ TCID₅₀·mL^-1 virus solution with a concentration of 0.2 mL per mouse was infected, and the mortality rate on the 6th day was 100%. HE staining of brain tissue showed lymphocyte infiltration. Pathogenicity test of piglets proved that there were no deaths although there were obvious symptoms. A strain of porcine pseudorabies virus was successfully isolated, which had the highest homology with the vaccine strain Bartha-K61, which showed enhanced pathogenicity in mice and lower pathogenicity in piglets. This study provides a reference for the genetic evolution analysis and epidemiological study of PRV in Shandong Province.

Key words: pseudorabies virus, separation and identification, genetic variation, pathogenicity

中图分类号:

S852.659.1

胡文佳, 时建皓, 李佳妮, 殷雨晨, 张新月, 董海冰, 杨海燕, 张传美. 一株猪伪狂犬病病毒Bartha-K61疫苗变异株的分离鉴定及生物学特性分析[J]. 畜牧兽医学报, 2025, 56(12): 6293-6298.

HU Wenjia, SHI Jianhao, LI Jiani, YIN Yuchen, ZHANG Xinyue, DONG Haibing, YANG Haiyan, ZHANG Chuanmei. Isolation, Identification and Biological Characterization of a Porcine Pseudorabies Virus Bartha-K61 Vaccine Variant[J]. Acta Veterinaria et Zootechnica Sinica, 2025, 56(12): 6293-6298.

图/表 3

参考文献 13

1	FREULING C M , MULLER T F , METTENLEITERTC . Vaccines against pseudorabies virus(PrV)[J]. Vet Microbiol, 2017, 206, 3- 9. doi: 10.1016/j.vetmic.2016.11.019
2	LIU Q Y , KUANG Y , LI Y F , et al. The epidemiology and variation in pseudorabies virus: A continuing challenge to pigs and humans[J]. Viruses, 2022, 14 (7): 1463. doi: 10.3390/v14071463
3	POMERANZ L E , REYNOLDS A E , HENGARTNER C J . Molecular biology of pseudorabies virus: impact on neurovirology and veterinary medicine[J]. Microbiol Mol Biol Rev, 2005, 69 (3): 462- 500. doi: 10.1128/MMBR.69.3.462-500.2005
4	MORIAH L S , YOLANDA R T , LANCE P , et al. A wide extent of inter-strain diversity in virulent and vaccine strains of alphaherpesviruses[J]. PLoS Pathog, 2011, 7 (10): e1002282. doi: 10.1371/journal.ppat.1002282
5	LI X D , YANG F L , HU X L , et al. Two classes of protective antibodies against pseudorabies virus variant glycoprotein B: Implications for vaccine design[J]. PLoS Pathog, 2017, 13 (12): e1006777. doi: 10.1371/journal.ppat.1006777
6	李佳轩. 伪狂犬病毒变异株的分离鉴定[D]. 长春: 吉林大学, 2018.
	LI J X. Isolation and identification of a mutated pseudorabies virus[D]. Changchun: Jilin University, 2018. (in Chinese)
7	LI A , LU G W , QI J X , et al. Structural basis of nectin-1 recognition by pseudorabies virus glycoprotein D[J]. PLoS Pathog, 2017, 13 (5): e1006314. doi: 10.1371/journal.ppat.1006314
8	陈超. 山东省免疫猪场猪伪狂犬病毒分离鉴定及gE、gC、gD和TK基因的序列分析[D]. 泰安: 山东农业大学, 2016.
	CHEN C. Sequence analysis of gE, gC, gD and TK gene among pseudorabies Ⅰ virus strains[D]. Taian: Shandong Agricultural University, 2016. (in Chinese)
9	任庆海. Bartka K61和变异株疫苗对猪伪狂犬病病毒变异株的免疫保护与猪伪狂犬病综合防控措施的研究[D]. 扬州: 扬州大学, 2022.
	REN Q H. Efficacy of Bartha K61 and rPRV XJ5-gI-/gE-/TK- prototype vaccine against emerging vPRV XJ5 strain lethal challenge and development of comprehensive control measures against porcine pseudorabies[D]. Yangzhou: Yangzhou University, 2022. (in Chinese)
10	YE C , ZHANG Q , TIAN Z , et al. Corrigendum to "Genomic characterization of emergent pseudorabies virus in China reveals marked sequence divergence: Evidence for the existence of two major genotypes"[J]. Virology, 2015, 483, 32- 43. doi: 10.1016/j.virol.2015.04.013
11	HE W T , AUCLERT L Z , ZHAI X F , et al. Interspecies transmission, genetic diversity, and evolutionary dynamics of pseudorabies virus[J]. J Infect Dis, 2019, 219 (11): 1705- 1715. doi: 10.1093/infdis/jiy731
12	石谦. 猪细小病毒-伪狂犬病重组病毒活疫苗SA215(B)的研究[D]. 成都: 四川农业大学, 2006.
	SHI Q. Study on porcine parvovirus and pseudorabies virus reeombinant live vaccine SA215(B)[D]. Chengdu: Sichuan Agricultural University, 2006. (in Chinese)
13	REN J L , WANG H B , ZHOU L , et al. Glycoproteins C and D of PRV strain HB1201 contribute individually to the escape from Bartha-K61 vaccine-induced immunity[J]. Front Microbiol, 2020, 11323.

[1]	陆灵光, 刘家齐, 卢佩琪, 吴瑞彬, 黄丹妮, 刘佳悦, 梁婉琪, 琚春梅. gD基因插入增强gI/gE/gM缺失重组伪狂犬病病毒对小鼠的免疫效果[J]. 畜牧兽医学报, 2026, 57(1): 327-336.
[2]	蔡扩军, 徐敏, 孙鹏亮, 夏利宁, 赵红琼. 新疆地区奶牛乳腺炎肺炎克雷伯菌的分离鉴定与耐药性分析[J]. 畜牧兽医学报, 2026, 57(1): 464-475.
[3]	赵秀美, 赵海娜, 华姝, 程旭, 陈祥, 姜逸. 冠状病毒辅助蛋白功能研究进展[J]. 畜牧兽医学报, 2026, 57(1): 96-107.
[4]	余秋蓉, 蔡旭航, 何艺, 李基棕, 毛立, 许信刚, 李彬. 一株羊冠状病毒的分离鉴定及全基因组序列分析[J]. 畜牧兽医学报, 2025, 56(9): 4604-4614.
[5]	曾圣鑫, 宋承琦, 申恺源, 郝国鑫, 王雅琨, 王鑫, 王晓旭, 刘志杰, 刘永波, 刘永生, 杨顺利, 付志新. 梅花鹿源A型产气荚膜梭菌的分离鉴定及对小鼠的致病性分析[J]. 畜牧兽医学报, 2025, 56(8): 3976-3984.
[6]	田茹, 付星玮, 胡乐玉, 朱明君, 童德文. 一株GⅡa型猪流行性腹泻病毒的分离与致病性分析[J]. 畜牧兽医学报, 2025, 56(8): 4101-4111.
[7]	向令娴, 季倩宇, 单新新, 李琳. 细菌双组分系统的耐药性及致病性研究进展[J]. 畜牧兽医学报, 2025, 56(7): 3116-3128.
[8]	王妍, 黄焮榆, 吴桂莹, 吴婉萍, 吕其壮. 2017—2023年我国猪圆环病毒3型的遗传变异分析[J]. 畜牧兽医学报, 2025, 56(6): 2857-2867.
[9]	陈云龙, 樊港, 樊心怡, 郭永超, 张鑫淼, 王妍, 张仕强. 一株具有多位点核苷酸替换的新型猪圆环病毒2d亚型毒株的分离与鉴定[J]. 畜牧兽医学报, 2025, 56(6): 3032-3040.
[10]	王亚楠, 郭雅茹, 姜艳平, 崔文, 李佳璇, 李一经, 王丽. 猪轮状病毒的分离鉴定及其致病性分析[J]. 畜牧兽医学报, 2025, 56(5): 2259-2269.
[11]	王锦祥, 苏进博, 付环茹, 孙世坤, 高承芳, 陈冬金, 桑雷, 谢喜平. 兔源A型多杀性巴氏杆菌Pm3和Pm6的致病性和基因组特征分析[J]. 畜牧兽医学报, 2025, 56(5): 2340-2352.
[12]	罗诗师, 陈蓓蕾, 张蕾, 冯启贤, 吴瑞森, 陈佳祺, 王媛, 简子昕, 许丽惠, 陈秋勇, 马玉芳, 王全溪. 太子参多糖经Let-7d-3p下调伪狂犬病病毒感染小鼠的炎症基因转录水平[J]. 畜牧兽医学报, 2025, 56(5): 2438-2450.
[13]	葛雷, 仇汝龙, 范志宇, 胡波, 魏后军, 陈萌萌, 宋艳华, 李一鸣, 徐为中, 王芳. 鼠伤寒沙门菌ompW基因缺失株与回补株的构建及其生物学特性[J]. 畜牧兽医学报, 2025, 56(4): 1865-1875.
[14]	范杰, 邰易润, 朱艳丽, 陈志雄, 胡巧云, 陈智, 刘甜甜, 李欣, 范仲鑫, 葛猛. 近年湖南省猪圆环病毒2型感染状况的调查及遗传演化分析[J]. 畜牧兽医学报, 2025, 56(3): 1376-1385.
[15]	张营营, 郭佳业, 许会艳, 毋亚运, 吴龙飞, 孙嵩英, 赵文超, 张龙现, 张素梅, 李俊强. 贾第虫对长爪沙鼠的致病性分析[J]. 畜牧兽医学报, 2025, 56(3): 1408-1418.

一株猪伪狂犬病病毒Bartha-K61疫苗变异株的分离鉴定及生物学特性分析

Isolation, Identification and Biological Characterization of a Porcine Pseudorabies Virus Bartha-K61 Vaccine Variant

PDF

可视化

摘要/Abstract

引用本文

使用本文

图/表 3

参考文献 13

相关文章 15

编辑推荐

Metrics

本文评价