丝胶蛋白对承德无角黑山羊精液冷冻保存的影响

doi:10.11843/j.issn.0366-6964.2024.10.019

摘要/Abstract

摘要：

旨在探究丝胶蛋白对承德无角黑山羊精液冷冻保存效果的影响。本试验采用假阴道法采集3只健康状态良好的承德无角黑山羊精液，随机分为6组，分别为对照组和在OptiXcell 2稀释液中添加不同浓度的(0.2%、0.4%、0.6%、0.8%、1%)丝胶蛋白试验组，每组设置5个重复，精液稀释完后进行细管分装、平衡、冷冻；精液冷冻-解冻后检测精子活力、顶体完整率、质膜完整率、总抗氧化能力(T-AOC)、谷胱甘肽过氧化物酶(GSH-Px)、超氧化歧化酶(SOD)、过氧化氢酶(CAT)活性、丙二醛(MDA)含量、线粒体膜电位、ATP含量等指标，并进行数据统计。结果显示，承德无角黑山羊精液冷冻稀释液中添加不同浓度丝胶蛋白均能够提高冻融后精子质量。其中，添加浓度为0.6%丝胶蛋白与其他各浓度相比，可显著提高精子冷冻-解冻后的精子活力、顶体完整率、质膜完整率以及线粒体膜电位和ATP含量，并且使抗氧化酶T-AOC、GSH-Px、SOD、CAT活性分别提高至(13.61±0.39) mmol·g^-1、(26.52±1.30) U·mg^-1、(165.45±11.10) U·mg^-1、(13.76±0.30) U·mg^-1，降低MDA值至(3.15±0.20) μmol·mg^-1。综上，在冷冻稀释液中添加不同浓度的丝胶蛋白均可提高承德无角黑山羊冷冻精液品质，且最适添加浓度为0.6%。

关键词: 丝胶蛋白, 承德无角黑山羊, 抗氧化剂, 精液冷冻

Abstract:

The aim of this study was to explore the effect of sericin on cryopreservation of semen of Chengde hornless black goat. Semen samples of 3 healthy Chengde hornless black goats were collected by the sham vaginal method and randomly divided into 6 groups: control group and experimental groups with different sericin concentrations (0.2%, 0.4%, 0.6%, 0.8%, 1%) added to the OptiXcell 2 dilution. Each group was set up with 5 replicates. Sperm motility, acrosome integrity rate, plasma membrane integrity rate, total antioxidant capacity (T-AOC), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), catalase (CAT) activity, malondialdehyde (MDA) content, mitochondrial membrane potential (MMP) and ATP content were detected after freezing and thawing. The results showed that the addition of different concentrations of sericin to the semen frozen dilution of Chengde hornless black goat could improve the quality of sperm after freezing and thawing. Compared with other concentrations, the addition of 0.6% sericin significantly increased sperm motility, acrosome integrity rate, plasma membrane integrity rate, mitochondrial membrane potential and ATP content after sperm freezing and thawing, and increased the activities of antioxidant enzymes T-AOC, GSH-Px, SOD and CAT to (13.61±0.39) mmol·g^-1, (26.52±1.30) U·mg^-1, (165.45±11.10) U·mg^-1 and (13.76 ±0.30) U·mg^-1, respectively. Meanwhile, the MDA value was reduced to (3.15±0.20) μmol·mg^-1. In summary, sericin at different concentrations can improve the quality of frozen semen of Chengde hornless black goat, and the most suitable concentration is 0.6%.

Key words: sericin, Chengde hornless black goat, antioxidants, semen freezing

中图分类号:

S827.3

张文涛, 于洋, 齐雅天, 赵炜, 张洪军, 陶晨雨, 夏威, 李俊杰. 丝胶蛋白对承德无角黑山羊精液冷冻保存的影响[J]. 畜牧兽医学报, 2024, 55(10): 4466-4474.

Wentao ZHANG, Yang YU, Yatian QI, Wei ZHAO, Hongjun ZHANG, Chenyu TAO, Wei XIA, Junjie LI. Effect of Sericin on Cryopreservation of Semen of Chengde Hornless Black Goat[J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(10): 4466-4474.

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丝胶蛋白浓度/% Sericin concentration	精子活力/% Sperm viability
0	42.39±1.23^e
0.2	45.66±1.87^cd
0.4	50.22±1.33^b
0.6	54.42±1.33^a
0.8	47.12±1.17^c
1	44.15±1.49^d

丝胶蛋白浓度/% Sericin concentration	总抗氧化能力/(mmol·g^-1) T-AOC	谷胱甘肽过氧化物酶/ (U·mg^-1) GSH-Px	丙二醛/ (μmol·mg^-1) MDA	超氧化歧化酶/ (U·mg^-1) SOD	过氧化氢酶/ (U·mg^-1) CAT
0	5.90±0.14^c	18.34±1.38^d	7.01±0.52^e	105.12±10.56^e	3.64±0.20^d
0.2	7.48±0.23^c	21.66±1.37^c	5.46±0.36^d	121.34±9.94^d	7.14±0.16^c
0.4	10.09±0.45^b	24.75±1.22^b	3.73±0.12^b	140.87±10.45^b	9.46±0.33^b
0.6	13.61±0.39^a	26.52±1.30^a	3.15±0.20^a	165.45±11.10^a	13.76±0.30^a
0.8	12.23±0.67^a	23.03±0.10^c	3.92±0.26^b	138.12±10.37^bc	9.54±0.20^b
1	8.90±0.33^b	22.84±1.13^c	4.88±0.14^c	128.41±7.27^cd	7.10±0.18^c

丝胶蛋白浓度/% Sericin concentration	ATP/(μmol·mL^-1) ATP content
0	7.00±0.33^d
0.2	10.87±0.46^c
0.4	13.26±0.24^b
0.6	15.31±0.52^a
0.8	13.46±0.27^b
1	11.18±0.32^c

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