尘螨诱导特应性皮炎小鼠模型和哮喘小鼠模型的构建

doi:10.11843/j.issn.0366-6964.2024.03.037

摘要/Abstract

摘要： 旨在构建尘螨诱导的特应性皮炎（atopic dermatitis， AD）小鼠模型和哮喘小鼠模型。本研究利用3种主要尘螨螨种（屋尘螨、粉尘螨、热带无爪螨）的主要变应原Der p 1、Der f 1、Blo t 5等比混合，分别诱导AD小鼠模型和哮喘小鼠模型，并通过皮肤及肺组织的相关炎症细胞因子水平（IFN-γ、IL-4、IL-10、IL-17A）、血清总IgE水平等指标对AD小鼠的过敏程度进行评估；通过IgE水平、耳朵点刺试验、肺部组织苏木精-伊红染色（hematoxylin-eosin staining，HE）等指标评估哮喘小鼠模型。结果表明，AD模型中致敏区域皮肤出现破损后结痂现象，皮肤及肺组织中细胞因子水平的变化表明小鼠体内反应偏向Th2反应，IgE水平较正常组显著升高；哮喘模型组小鼠血清总IgE水平显著升高，变应原激发后模型小鼠体温下降明显，且90 min后仍未恢复初始温度，耳朵点刺试验中，抗原的攻击使模型组小鼠耳朵染料渗漏增加，肺组织HE染色显示模型组小鼠炎症细胞浸润增加。本研究成功诱导尘螨AD小鼠模型和尘螨哮喘小鼠模型。

关键词: 特应性皮炎, 哮喘, 细胞因子, 动物模型

Abstract: The aims of this paper were to construct a mouse model of dust mite induced atopic dermatitis (AD) and a mouse model of asthma. In this experiment, the main allergens Der p 1, Der f 1, and Blo t 5 of the three main dust mite species (Dermatophagoides pteronyssinus, Dermatophagoides farinae, and Blomia tropicalis) were mixed in equal ratios to induce an AD mouse model and an asthma mouse model, respectively. The degree of sensitization in AD mice was also assessed by the levels of relevant inflammatory cytokines (IFN-γ, IL-4, IL-10, IL-17A) in skin and lung tissues, and total IgE serum levels. IgE levels, ear prick test, and hematoxylin-eosin staining of lung tissue (HE) were used to assess the asthma mouse model. The results showed that the skin of the sensitized area in the AD model showed crusting after breakage, and the changes of cytokine levels in the skin and lung tissues indicated that the in vivo response of the mice was in favor of Th2 response, and the IgE level was significantly higher than that of the normal group; The total serum IgE level was significantly increased in the mice of the asthma model group. The body temperature of the model mice decreased significantly after allergen excitation and did not recover the initial temperature after 90 min. In the ear prick test, the antigen attack increased the leakage of ear dye in the model mice, and the HE staining of lung tissue shows increased inflammatory cell infiltration in the model group of mice. This experiment successfully induced dust mite AD mouse model and dust mite asthma mouse model.

Key words: atopic dermatitis, asthma, cytokines, animal models

中图分类号:

罗承慧, 高江瑞, 陈俊威, 魏春洁, 韦双双, 裴业春. 尘螨诱导特应性皮炎小鼠模型和哮喘小鼠模型的构建[J]. 畜牧兽医学报, 2024, 55(3): 1257-1267.

LUO Chenghui, GAO Jiangrui, CHEN Junwei, WEI Chunjie, WEI Shuangshuang, PEI Yechun. Construction of Mouse Model of Dust Mite Induced Atopic Dermatitis and Asthma[J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(3): 1257-1267.

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