蒙古马和纯血马免疫相关基因差异表达分析

doi:10.11843/j.issn.0366-6964.2023.12.013

畜牧兽医学报 ›› 2023, Vol. 54 ›› Issue (12): 5020-5032.doi: 10.11843/j.issn.0366-6964.2023.12.013

蒙古马和纯血马免疫相关基因差异表达分析

任秀娟¹, 康德措², 苏少锋³, 蔺雅楠¹, 李雅静¹, 杜明¹, 白东义¹, 李蓓¹, 赵一萍^1*, 芒来^1*

1. 内蒙古农业大学动物科学学院/马属动物研究中心, 呼和浩特 010018;
2. 青海省种羊繁育推广服务中心, 海北藏族自治州 812300;
3. 内蒙古自治区农牧业科学院, 呼和浩特 010031

收稿日期:2023-07-18 出版日期:2023-12-23 发布日期:2023-12-26
通讯作者: 赵一萍,主要从事蒙古马肠道微生物及抗病性研究,E-mail:yipingzhao@imau.edu.cn;芒来,主要从事马属动物遗传育种与繁殖研究,E-mail:dmanglai@163.com
作者简介:任秀娟(1989-),女,内蒙古集宁人,博士,主要从事马属动物遗传育种与繁殖的研究,E-mail:renxiujuanimau@sina.com
基金资助:
内蒙古自治区科技重大专项(2020ZD0004);国家自然科学基金(31960657);内蒙古自治区重大项目(2021ZD0018);内蒙古农业大学动物科学学院项目(QN202103)

Comparison of Immune Gene Expression between Mongolian Horse and Thoroughbred

REN Xiujuan¹, KANG Decuo², SU Shaofeng³, LIN Yanan¹, LI Yajing¹, DU Ming¹, BAI Dongyi¹, LI Bei¹, ZHAO Yiping^1*, DUGARJAVIIN Manglai^1*

1. College of Animal Science/Equus Research Center, Inner Mongolia Agricultural University, Hohhot 010018, China;
2. Qinghai Sheep Breeding and Extension Service Center, Tibetan Autonomous Prefecture of Haibei 812300, China;
3. Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences, Hohhot 010031, China

Received:2023-07-18 Online:2023-12-23 Published:2023-12-26

摘要/Abstract

摘要： 为了评价蒙古马(Equus mongolian horse)和纯血马(E. thoroughbred)免疫生理机能在适应特殊生存环境和人工选择压力过程中相关基因的表达多样性。本研究对健康成年蒙古马(n=3)和纯血马(n=3)的颈静脉血液进行RNA-Seq和品种间表达谱比较分析。对2岁左右健康蒙古马(n=3)和纯血马(n=3)采集肝脏、脾脏、肺脏和血液样品,利用qRT-PCR方法,分析NF-κB信号通路7个基因在两个品种中的表达情况。利用Western blot方法,分析蒙古马和纯血马肺脏中核因子κB亚基p65 (RELA proto-oncogene NF-kB subunit, NF-κB p65)蛋白和白细胞介素1β (interleukin 1 beta, IL-1β)蛋白的表达情况。结果表明,RNA-Seq共获得19.69~23.95 Gb的高质量数据和1 199个差异表达基因,许多差异表达基因直接参与机体的免疫过程。NF-κB信号通路7个基因在蒙古马和纯血马4个免疫组织中均表达,基因表达的品种间差异存在组织多样性;7个基因在蒙古马肺脏的表达量均高于纯血马,除肿瘤坏死因子(tumor necrosis factor, TNF-α)基因差异不显著外(P≥0.05),其余基因均具有生物学统计意义(P<0.05)。NF-κB p65蛋白和IL-1β蛋白在蒙古马肺脏的表达量高于纯血马(P<0.05)。许多免疫相关基因在蒙古马和纯血马免疫组织之间差异表达,可能和蒙古马适应特定环境以及纯血马的运动性能定向选育有关。本研究结果为进一步研究蒙古马免疫功能适应特定环境的分子机制提供数据支持。

关键词: 蒙古马, 纯血马, 免疫相关基因, NF-κB信号通路

Abstract: The purposes of this study were to evaluate the variations in gene expression between Mongolian horse and Thoroughbred, which underlying the coordinate changes in the immunophysiological responses to the specific environmental conditions and directional breeding selection pressure. In this study, jugular blood collected from healthy adult Mongolian horses (n=3) and Thoroughbred (n=3) was used to perform RNA-Seq and comparative analysis of the expression profiles between two horse breeds. Liver, spleen, lung, and jugular blood samples were collected from about 2-year-old healthy Mongolian horses (n=3) and Thoroughbred (n=3). qRT-PCR was used to analyze the relative expression of 7 genes of NF-κB signaling pathway in liver, spleen, lung and blood tissues between two horse breeds. Western blot was used to analyze the relative expression of RELA proto-oncogene NF-kB subunit (NF-κB p65) protein and interleukin 1 beta (IL-1β) protein in the lungs between Mongolian horse and Thoroughbred. The results showed that 19.69-23.95 Gb high-quality RNA-Seq data were obtained and 1 199 differentially expressed genes were identified. Functional analysis showed that many differentially expressed genes directly participated in the immune process. The qRT-PCR results showed that 7 genes of NF-κB signaling pathway were expressed in 4 tissues of Mongolian horse and Thoroughbred, but the difference in gene expression between breeds was manifested as tissue diversity. The expression of 7 genes in the lung of Mongolian horse was higher than that of Thoroughbred, excepting for tumor necrosis factor (TNF-α) (P≥0.05), while all other genes had biological statistical significance (P<0.05). The expression of NF-κB p65 protein and IL-1β protein in the lung of Mongolian horse was also higher than that of Thoroughbred (P<0.05). Many immune-related genes were differentially expressed in the immune tissues between Mongolian horses and Thoroughbred, which might be due to the adaptation of Mongolian horses to harsh environments and the sports performance directional breeding of Thoroughbred. The results of this study provided data support for further research on the molecular mechanism of the Mongolian horse immune function adapting to specific environment.

Key words: Mongolian horse, Thoroughbred, immune-related gene, NF-κB signaling pathway

中图分类号:

S821.2

任秀娟, 康德措, 苏少锋, 蔺雅楠, 李雅静, 杜明, 白东义, 李蓓, 赵一萍, 芒来. 蒙古马和纯血马免疫相关基因差异表达分析[J]. 畜牧兽医学报, 2023, 54(12): 5020-5032.

REN Xiujuan, KANG Decuo, SU Shaofeng, LIN Yanan, LI Yajing, DU Ming, BAI Dongyi, LI Bei, ZHAO Yiping, DUGARJAVIIN Manglai. Comparison of Immune Gene Expression between Mongolian Horse and Thoroughbred[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(12): 5020-5032.

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蒙古马和纯血马免疫相关基因差异表达分析

Comparison of Immune Gene Expression between Mongolian Horse and Thoroughbred

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