生产母兔沙门菌感染的病理学变化及其药物敏感性分析

doi:10.11843/j.issn.0366-6964.2023.08.035

摘要/Abstract

摘要： 为探究生产母兔沙门菌感染的组织病理变化特征，本研究对河南某规模化兔场6只精神沉郁、呼吸急促、流产母兔剖检，见子宫内膜出血，内有死胎，心、肝、脾、肺、肾表面充血淤血。经病原菌分离培养、形态观察、血清分型、PCR鉴定、测序分析及致病性试验鉴定，确定分离病原菌为沙门菌。通过对心、肝、脾、肺、肾的组织病理学观察，发现病兔心肌纤维弯曲、排列紊乱，肝组织颗粒或脂肪变性，肺组织淤血，子宫化脓、子宫内膜炎，以及其他器官充血。药物敏感性试验结果显示，病原菌对氯霉素类、头孢菌素类、青霉素类、喹诺酮类、四环素类、多肽类、β-内酰胺、磺胺类抗生素具有一定的敏感性，对氨基糖苷类（丁胺卡那）有一定的耐药性。本研究成功分离到兔沙门菌，并对其感染母兔进行组织病理学与药物敏感性分析，为更好地预防和控制兔沙门菌病提供依据。

关键词: 规模化兔场, 生产母兔, 沙门菌感染, 病理学, 药敏分析

Abstract: In order to explore the histopathological characteristics of Salmonella infection in female rabbits, 6 dead female rabbits with depressed, shortness of breath and aborted from a large-scale rabbit farm in Henan province were examined. Endometrial bleeding, stillbirth, and hyperemia and congestion on the surface of heart, liver, spleen, lung and kidney were found by anatomical examination. The isolated pathogen was identified as Salmonella by isolation culture, morphological observation, serological typing, PCR identification, sequencing analysis and pathogenicity test. It was found that the myocardial fibers were bent or arranged disordered, liver cell granular degeneration or steatosis, lung extensive congestion, suppurative hysteritis, endometritis and other organs hyperemia by histopathological observation. The results of drug sensitivity test showed that the pathogens were sensitive to chloramphenicol, cephalosporins, penicillins, quinolones, tetracyclic, polypeptide, β-lactam and sulfa antibiotics, and had certain resistance to aminoglycoside (butamicana). In this study, Salmonella was successfully isolated from rabbits, the histopathological and drug sensitivity analysis of the infected female rabbits were carried out, which provides a basis for better prevention and control of rabbit salmonellosis.

Key words: large-scale rabbit farm, productive female rabbits, Salmonella infection, pathology, susceptibility analysis

中图分类号:

S858.2915.1

张倩文, 刘玉梅, 石丽辉, 梁文军, 李梦云, 王玉琴, 张自强. 生产母兔沙门菌感染的病理学变化及其药物敏感性分析[J]. 畜牧兽医学报, 2023, 54(8): 3510-3518.

ZHANG Qianwen, LIU Yumei, SHI Lihui, LIANG Wenjun, LI Mengyun, WANG Yuqin, ZHANG Ziqiang. Pathological Observation and Drug Sensitivity Analysis of Salmonella Infection in Female Rabbits[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3510-3518.

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