番茄红素对壬基酚诱导小鼠脾损伤的保护作用及机制研究

doi:10.11843/j.issn.0366-6964.2022.02.030

畜牧兽医学报 ›› 2022, Vol. 53 ›› Issue (2): 637-645.doi: 10.11843/j.issn.0366-6964.2022.02.030

番茄红素对壬基酚诱导小鼠脾损伤的保护作用及机制研究

杨少青^1,2,3, 邵春艳^1,2,3, 周彬^1,2,3, 宋泉江^1,2,3, 王晓杜^1,2,3*, 宋厚辉^1,2,3*, 姜胜^1,2,3*

1. 浙江农林大学动物科技学院、动物医学院, 杭州 311300;
2. 浙江省畜禽绿色生态健康养殖应用技术研究重点实验室, 杭州 311300;
3. 动物健康互联网检查技术浙江省工程实验室, 杭州 311300

收稿日期:2021-03-24 出版日期:2022-02-23 发布日期:2022-03-02
通讯作者: 姜胜,主要从事动物麻醉与镇痛研究,E-mail:cjsheng@zafu.edu.cn;王晓杜,主要从事动物病毒学研究,E-mail:xdwang@zafu.edu.cn;宋厚辉,主要从事动物疫病诊断研究,E-mail:songhh@zafu.edu.cn
作者简介:杨少青(1996-),女,浙江温州人,硕士生,主要从事畜禽毒理与内科疾病研究,E-mail:1051672039@qq.com
基金资助:
国家自然科学基金（31602119）；浙江省重点实验室开放项目（S2019018）；浙江农林大学科研启动项目（2015FR042）

Protective Effect and Mechanism of Lycopene on Spleen Injury Induced by Nonylphenol in Mice

YANG Shaoqing^1,2,3, SHAO Chunyan^1,2,3, ZHOU Bin^1,2,3, SONG Quanjiang^1,2,3, WANG Xiaodu^1,2,3*, SONG Houhui^1,2,3*, JIANG Sheng^1,2,3*

1. College of Animal Science and Technology·College of Veterinary Medicine, Zhejiang A&F University, Hangzhou 311300, China;
2. Key Laboratory of Applied Technology on Green-Eco-Healthy Animal Husbandry of Zhejiang Province, Hangzhou 311300, China;
3. Zhejiang Provincial Engineering Laboratory for Animal Health Inspection and Internet Technology, Hangzhou 311300, China

Received:2021-03-24 Online:2022-02-23 Published:2022-03-02

摘要/Abstract

摘要： 探讨番茄红素（lycopene，LYC）对壬基酚（nonylphenol，NP）亚慢性中毒诱导小鼠脾损伤的保护作用及机制。将40只清洁级ICR小鼠随机分为对照组（CON）、中毒组（NPG）、番茄红素对照组（LCG）和番茄红素干预组（LNG）。对照组，每天8：30灌服玉米油0.1 mL，2 h后重复操作；中毒组，每天8：30灌服玉米油0.1 mL，2 h后灌服150 mg·kg^-1壬基酚溶液；番茄红素对照组，每天8：30灌服5 mg·kg^-1番茄红素，2 h后灌服0.1 mL玉米油；番茄红素干预组，每天8：30灌服5 mg·kg^-1番茄红素，2 h后灌服150 mg·kg^-1壬基酚溶液。各组连续进行相应灌胃处理30 d后，记录小鼠体重，颌下静脉采集血样，并对小鼠进行安乐死，剖解后采集小鼠脾称重，分析小鼠日均增重以及脾体系数的组间差异；检查血清中免疫细胞因子IL-2、IFN-γ和TNF-α的变化；检测脾氧化与抗氧化指标MDA、T-AOC、CAT、GSH-Px和SOD的差异变化；检测凋亡通路p53/Bcl-2各蛋白表达的差异。结果表明，与NPG相比，经过LYC干预后NP暴露小鼠日均增重显著升高（P<0.05），血清免疫细胞因子含量和脾抗氧化酶活性显著升高（P<0.05）；LYC干预后小鼠脾凋亡通路关键分子Akt、p-Akt和Bcl-2蛋白表达水平显著升高（P<0.05），p53和Bax蛋白表达水平显著降低（P<0.05）。结果提示，LYC对NP诱导的小鼠脾损伤具有保护作用，这与LYC增强脾抗氧化能力，抑制脾细胞凋亡有关。

关键词: 番茄红素, 壬基酚, 氧化应激, 凋亡

Abstract: The study aimed to investigate the protective effect and mechanism of lycopene (LYC) on subchronic poisoning of nonylphenol (NP) in spleen of mice. Forty ICR mice were randomly divided into four groups including control group (CON), poisoning group (NPG), lycopene control group (LCG) and lycopene intervention group (LNG). Mice in CON were given 0.1 mL corn oil at 8:30 every day, and the operation was repeated after two hours; mice in NPG were administrated 0.1 mL corn oil at 8:30 every day, and then were given 150 mg·kg^-1 NP two hours later; mice in LCG were given 5 mg·kg^-1 LYC at 8:30 every day, and then were given 0.1 mL corn oil two hours later; mice in LNG were given 5 mg·kg^-1 LYC at 8:30 every day, and then were administrated 150 mg·kg^-1 NP two hours later. After 30 days of consecutive gavage, the weight of mice was recorded, blood samples were collected from submaxillary vein, then mice were euthanized, and the spleen of mice was collected and weighed after dissection, and the difference between the groups of weight growth rate and spleen body coefficient were analyzed. The changes of immunity cytokines IL-2, IFN-γ and TNF-α in serum were examined, and the spleen oxidation and anti oxidative indexes MDA, T-AOC, CAT, GSH-Px and SOD was observed, and the protein expression in p53/Bcl-2 apoptosis pathway was detected. The results showed that compared with NPG, the weight growth rate of mice was significantly increased after LYC treatment (P<0.05), serum cytokines and spleen antioxidant enzyme activity were significantly increased (P<0.05); the expression levels of key molecules of apoptosis pathway Akt, p-Akt and Bcl-2 protein in spleen of mice after LYC treatment were significantly increased (P<0.05), and the expression level of p53 and Bax protein was significantly decreased (P<0.05). LYC has protective effect on NP induced spleen injury in mice, which was related to enhancing the anti-oxidation ability of spleen and inhibiting apoptosis of spleen cells.

Key words: lycopene, nonylphenol, oxidative stress, apoptosis

中图分类号:

S852.4

杨少青, 邵春艳, 周彬, 宋泉江, 王晓杜, 宋厚辉, 姜胜. 番茄红素对壬基酚诱导小鼠脾损伤的保护作用及机制研究[J]. 畜牧兽医学报, 2022, 53(2): 637-645.

YANG Shaoqing, SHAO Chunyan, ZHOU Bin, SONG Quanjiang, WANG Xiaodu, SONG Houhui, JIANG Sheng. Protective Effect and Mechanism of Lycopene on Spleen Injury Induced by Nonylphenol in Mice[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(2): 637-645.

参考文献 29

[1]	EL-DAKDOKY M H,HELAL M A M.Reproductive toxicity of male mice after exposure to nonylphenol[J].Bull Environ Contam Toxicol,2007,79(2):188-191.
[2]	GRISOLIA C K,BILICH M R,FORMIGLI L M.A comparative toxicologic and genotoxic study of the herbicide arsenal,its active ingredient imazapyr,and the surfactant nonylphenol ethoxylate[J].Ecotox Environ Safe,2004,59(1):123-126.
[3]	PARK H J,ZHANG M T,LEE W Y,et al.Toxic effects of nonylphenol on neonatal testicular development in mouse organ culture[J].Int J Mol Sci,2020,21(10):3491.
[4]	KAZEMI S,MOUSAVI KANI S N,GHASEMI-KASMAN M,et al.Nonylphenol induces liver toxicity and oxidative stress in rat[J].Biochem Biophys Res Commun,2016,479(1):17-21.
[5]	KOTB A M,ABD-ELKAREEM M,ABOU KHALIL N S,et al.Protective effect of Nigella sativa on 4-nonylphenol-induced nephrotoxicity in Clarias gariepinus (Burchell,1822)[J].Sci Total Environ,2018,619-620:692-699.
[6]	MAO Z,ZHENG Y L,ZHANG Y Q.Behavioral impairment and oxidative damage induced by chronic application of nonylphenol[J].Int J Mol Sci,2011,12(1):114-127.
[7]	CHOI J S,OH J H,PARK H J,et al.Mirna regulation of cytotoxic effects in mouse Sertoli cells exposed to nonylphenol[J].Reprod Biol Endocrinol,2011,9:126.
[8]	COSTA-RODRIGUES J,FERNANDES M H,PINHO O,et al.Modulation of human osteoclastogenesis and osteoblastogenesis by lycopene[J].J Nutr Biochem,2018,57:26-34.
[9]	ALVI S S,ANSARI I A,AHMAD M K,et al.Lycopene amends LPS induced oxidative stress and hypertriglyceridemia via modulating PCSK-9 expression and Apo-CIII mediated lipoprotein lipase activity[J].Biomed Pharmacother,2017,96:1082-1093.
[10]	GRAFF R E,PETTERSSON A,LIS R T,et al.Dietary lycopene intake and risk of prostate cancer defined by ERG protein expression[J].Am J Clin Nutr,2016,103(3):851-860.
[11]	LI W S,JIANG B H,CAO X L,et al.Protective effect of lycopene on fluoride-induced ameloblasts apoptosis and dental fluorosis through oxidative stress-mediated caspase pathways[J].Chem Biol Interact,2017,261:27-34.
[12]	PALABIYIK S S,ERKEKOGLU P,ZEYBEK N D,et al.Protective effect of lycopene against ochratoxin A induced renal oxidative stress and apoptosis in rats[J].Exp Toxicol Pathol,2013,65(6):853-861.
[13]	PALOZZA P,SIMONE R,CATALANO A,et al.Lycopene prevents 7-ketocholesterol-induced oxidative stress,cell cycle arrest and apoptosis in human macrophages[J].J Nutr Biochem,2010,21(1):34-46.
[14]	XU F B,WANG P Y,YAO Q C,et al.Lycopene alleviates AFB1-induced immunosuppression by inhibiting oxidative stress and apoptosis in the spleen of mice[J].Food Funct,2019,10(7):3868-3879.
[15]	SRINIVASAN M,SUDHEER A R,PILLAI K R,et al.Lycopene as a natural protector against γ-radiation induced DNA damage,lipid peroxidation and antioxidant status in primary culture of isolated rat hepatocytes in vitro[J].Biochim Biophys Acta,2007,1770(4):659-665.
[16]	RAO A V,AGARWAL S.Role of lycopene as antioxidant carotenoid in the prevention of chronic diseases:a review[J].Nutr Res,1999,19(2):305-323.
[17]	ESTEBAN S,GORGA M,PETROVIC M,et al.Analysis and occurrence of endocrine-disrupting compounds and estrogenic activity in the surface waters of Central Spain[J].Sci Total Environ,2014,466-467:939-951.
[18]	SCHWAIGER J,SPIESER O H,BAUER C,et al.Chronic toxicity of nonylphenol and ethinylestradiol:haematological and histopathological effects in juvenile Common carp (Cyprinus carpio)[J].Aquat Toxicol,2000,51(1):69-78.
[19]	HOWELL M,SHEPHERD M.The immune system[J].Anaesth Intens Care Med,2018,19(10):575-578.
[20]	ZHU J F,PAUL W E.CD4 T cells:fates,functions,and faults[J].Blood,2008,112(5):1557-1569.
[21]	BERG R E,FORMAN J.The role of CD8 T cells in innate immunity and in antigen non-specific protection[J].Curr Opin Immunol,2006,18(3):338-343.
[22]	VALKO M,IZAKOVIC M,MAZUR M,et al.Role of oxygen radicals in DNA damage and cancer incidence[J].Mol Cell Biochem,2004,266(1-2):37-56.
[23]	IMLAY J A.Pathways of oxidative damage[J].Annu Rev Microbiol,2003,57:395-418.
[24]	MILANI A,BASIRNEJAD M,SHAHBAZI S,et al.Carotenoids:biochemistry,pharmacology and treatment[J].Br J Pharmacol,2017,174(11):1290-1324.
[25]	COSTA-RODRIGUES J,PINHO O,MONTEIRO P R R.Can lycopene be considered an effective protection against cardiovascular disease?[J].Food Chem,2018,245:1148-1153.
[26]	GHOBRIAL I M,WITZIG T E,ADJEI A A.Targeting apoptosis pathways in cancer therapy[J].CA Cancer J Clin,2005,55(3):178-194.
[27]	KLUCK R M,BOSSY-WETZEL E,GREEN D R,et al.The release of cytochrome c from mitochondria:a primary site for Bcl-2 regulation of apoptosis[J].Science,1997,275(5303):1132-1136.
[28]	GROSS A,MCDONNELL J M,KORSMEYER S J.BCL-2 family members and the mitochondria in apoptosis[J].Genes Dev,1999,13(15):1899-1911.
[29]	HAUPT S,BERGER M,GOLDBERG Z,et al.Apoptosis-the p53 network[J].J Cell Sci,2003,116(20):4077-4085.

番茄红素对壬基酚诱导小鼠脾损伤的保护作用及机制研究

Protective Effect and Mechanism of Lycopene on Spleen Injury Induced by Nonylphenol in Mice

RichHTML

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献 29

相关文章 15

编辑推荐

Metrics

本文评价

[1]	董书餐, 毛帅翔, 伍翠莹, 李耀坤, 孙宝丽, 郭勇庆, 邓铭, 刘德武, 柳广斌. 雄激素受体抑制剂恩杂鲁胺对山羊卵泡颗粒细胞增殖凋亡的影响[J]. 畜牧兽医学报, 2024, 55(5): 2022-2031.
[2]	王吉英, 尹蕊如, 谢星, 王海燕, 刘胡栋, 胡辉, 熊祺琰, 冯志新, 邵国青, 于岩飞. 猪肺炎支原体乳酸脱氢酶在诱导猪支气管上皮细胞凋亡中的作用[J]. 畜牧兽医学报, 2024, 55(5): 2195-2205.
[3]	李秋云, 田芯源, 廖文圣, 张焕容, 任玉鹏, 杨发龙, 朱江江, 向华. SOCS2对山羊鼻甲骨细胞增殖、周期及凋亡的影响[J]. 畜牧兽医学报, 2024, 55(5): 2226-2240.
[4]	蓝昕蕊, 赵宝宝, 张碧菡, 林晓语, 马会明, 王勇胜. β-谷甾醇对猪卵母细胞体外成熟和胚胎发育的影响[J]. 畜牧兽医学报, 2024, 55(4): 1629-1637.
[5]	章心婷, 邱文粤, 庞晓玥, 苏依曼, 叶嘉莉, 黄健佳, 周水莲, 唐兆新, 王荣梅, 苏荣胜. 积雪草酸通过抑制氧化应激和铁死亡减轻脂多糖诱导的肉鸡心肌损伤的研究[J]. 畜牧兽医学报, 2024, 55(4): 1787-1799.
[6]	姜丽君, 宗云鹤, 李云雷, 陈继兰, 耿照玉, 孙研研, 金四华. 抗氧化剂在家禽精液储存中的应用研究进展[J]. 畜牧兽医学报, 2024, 55(3): 913-923.
[7]	虎巧燕, 翟相钦, 李一丹, 韩家乐, 雷初朝, 党瑞华. Bta-miR-101对牛睾丸支持细胞增殖、凋亡及分泌的影响[J]. 畜牧兽医学报, 2024, 55(3): 1040-1051.
[8]	王鑫, 聂桐, 李阿群, 马隽. 橙皮苷通过氧化磷酸化途径缓解高脂饲喂诱导的小鼠肝氧化应激[J]. 畜牧兽医学报, 2024, 55(3): 1302-1313.
[9]	霍元楠, 邱美佳, 张姣姣, 杨炜蓉, 王鲜忠. 精氨酸及其代谢物抑制热应激诱导仔猪支持细胞凋亡的机制[J]. 畜牧兽医学报, 2024, 55(2): 587-597.
[10]	邱文粤, 苏依曼, 叶嘉莉, 章心婷, 庞晓玥, 王荣梅, 谢子茂, 张辉, 唐兆新, 苏荣胜. 积雪草酸通过调控细胞凋亡和自噬缓解脂多糖诱导肉鸡急性肾损伤的研究[J]. 畜牧兽医学报, 2024, 55(2): 809-821.
[11]	陈松彪, 刘飞飞, 尚珂, 余祖华, 何雷, 魏颖, 陈建, 张春杰, 程相朝, 丁轲. 病毒感染与宿主抗感染免疫之间“博弈”——凋亡、坏死和焦亡分子机制[J]. 畜牧兽医学报, 2024, 55(1): 59-70.
[12]	段香茹, 康佳, 杨若晨, 单新雨, 李太春, 赵雯, 张英杰, 刘月琴. L-半胱氨酸对绵羊卵巢颗粒细胞增殖、凋亡和类固醇激素分泌的影响[J]. 畜牧兽医学报, 2024, 55(1): 179-191.
[13]	陈鸿, 阮红日, 马天文, 李亚楠, 苗雪, 杨雯越, 高利, 魏成威. 葛根素干预软骨氧化应激和Nrf2/HO-1通路改善PTOA大鼠软骨退变的机制[J]. 畜牧兽医学报, 2023, 54(9): 3951-3963.
[14]	宋美君, 郝科兴, 海思妤, 陈岩, 王静, 胡广东. SRIF-14对绵羊子宫内膜上皮细胞增殖和凋亡的影响[J]. 畜牧兽医学报, 2023, 54(8): 3325-3334.
[15]	徐茜, 杨柏高, 张航, 冯肖艺, 郝海生, 杜卫华, 朱化彬, 张培培, 赵学明. β-烟酰胺单核苷酸对牛卵母细胞脂滴含量及冷冻效果的影响[J]. 畜牧兽医学报, 2023, 54(8): 3348-3357.