新疆地区3株驴源马链球菌马亚种新SeM基因型的鉴定与进化分析

doi:10.11843/j.issn.0366-6964.2022.01.023

摘要/Abstract

摘要： 从新疆地区某驴养殖场获得了3株驴腺疫链球菌分离株HTP133、HTP123和HTP232。为了解这3株驴腺疫链球菌的生物学特性和确定其分子分型，本研究对其进行了生化特性、药敏特性的检测，对16S rRNA进行序列对比分析，并利用PCR扩增SeM等位基因和测序鉴定其基因型。研究结果表明3株分离菌均为马链球菌马亚种。药敏试验结果显示，分离菌均对阿莫西林、氨苄西林和庆大霉素等18种药物敏感，对青霉素、头孢呋辛耐药。16S rRNA分析结果显示分离菌株归属于该菌16S rRNA进化群的Ⅰ群，且在V1-V5可变区有明显的序列特征，且与JQ726493株进化关系较近。SeM等位基因测定后鉴定了2个新SeM基因型，分别为SeM 210（HTP232）和SeM 211（HTP 133、HTP 123），与SeM 136、SeM 138、SeM 142、SeM 146、SeM 149和SeM 144亲缘关系较近。本研究丰富了我国驴腺疫链球菌的流行和传播特征，提供了相关的分子生物学基础，为驴腺疫的防控提供理论依据。

关键词: 驴源马链球菌马亚种, 16S rRNA分析, SeM分子分型, 药敏特性

Abstract: Three isolates of Streptococcus subsp. equi HTP133, HTP123 and HTP232 were obtained from a donkey farm in Xinjiang. To identify the genotypingand understand the biological and epidemic characteristics of these three isolates,the biochemical characteristics analysis,drug sensitivity test and 16S rRNA sequence comparison analysis and phylogenetic tree construction were performed. The SeM alleles were amplified by PCR and sequenced for genotyping. The results showed that these three isolates were Streptococcus equi subsp.equi. These three isolates were sensitive to 18 drugs, including amoxicillin, ampicillin and gentamicin etc, whereas they are resistant to penicillin and cefuroxime. Form the 16S rRNA gene analysis and the phylogentic tree, we found that these isolated strains belonged to group I and there is an obvious variation in their variable regions of V1-V5. The homology of these three isolates are closely related with S. equi JQ726493. In addition,two new SeM genotypes, SeM 210 (HTP232) and SeM 211 (HTP133, HTP123) were identified by SeM allele analysis and they were closely related to SeM 136, SeM 138, SeM 142 and SeM 146 genotype.This work enriches the molecular biological foundation of prevalence and transmission characteristics of donkey S. equi in China and provides the theoreitical basis for the prevention and control of donkey strangles as well.

Key words: donkey Streptococcus equi subsp. equi, 16S rRNA analysis, SeM genotyping, drug sensitivity

中图分类号:

S852.611

杜宇, 蒲小峰, 陈晓萌, 吕芬芬, 汪丽, 张泽华, 张宝江, 苏艳. 新疆地区3株驴源马链球菌马亚种新SeM基因型的鉴定与进化分析[J]. 畜牧兽医学报, 2022, 53(1): 231-240.

DU Yu, PU Xiaofeng, CHEN Xiaomeng, Lü Fenfen, WANG Li, ZHANG Zehua, ZHANG Baojiang, SU Yan. Identification and Evolutionary Analysis of Three Novel SeM Genotypes of Donkey Streptococcus equi subsp. equi Isolates in Xinjiang[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(1): 231-240.

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