中国部分地区犬源伪中间葡萄球菌的流行特征分析

doi:10.11843/j.issn.0366-6964.2021.09.019

摘要/Abstract

摘要： 旨在调查犬源伪中间葡萄球菌的耐药性和分子特征，本研究自2017年11月—2019年4月收集601份送检至中国农业大学动物医院检验科和第三方检测机构的犬源临床样本，分离鉴定伪中间葡萄球菌；采用琼脂稀释法检测伪中间葡萄球菌对14种抗菌药物的敏感性；所有分离菌株均进行全基因组测序（WGS），并从WGS结果中获取耐药基因、多位点序列分型（ST）和葡萄球菌染色体基因盒（SCCmec）分型。结果显示，共分离到75株伪中间葡萄球菌（分离率为12.5%），其中包括42株（56%）耐甲氧西林伪中间葡萄球菌（MRSP）和33株（44%）甲氧西林敏感伪中间葡萄球菌（MSSP）；所有菌株均对青霉素、阿奇霉素、克林霉素、多西环素、环丙沙星、恩诺沙星、苯唑西林和氯霉素高度耐药，对利奈唑胺、万古霉素、阿米卡星和利福平敏感，多重耐药率达90.7%；所有MRSP均具有多重耐药性；75株伪中间葡萄球菌中检测到19种耐药基因，blaZ和aac（6'）-aph（2″）检出率最高，均为92%（69/75）；共发现70种ST型，54种为新ST型，无优势ST型；23株（54.7%） MRSP为SCCmec V型。综上表明，犬源伪中间葡萄球菌存在严重的多重耐药性；MRSP和MSSP均具有较大的遗传多样性。

关键词: 犬, 伪中间葡萄球菌, 多重耐药性, 耐药基因, 分子分型

Abstract: This study aimed to investigate the antimicrobial resistance phenotype, genotype, and molecular characteristics of Staphylococcus pseudintermedius isolated from clinical samples in dogs. From November 2017 to April 2019, 601 samples were collected from the Veterinary Teaching Hospital of China Agricultural University (VTH-CAU) and third-party testing organizations, and identified by PCR amplification of 16S rDNA gene sequencing. Antimicrobial susceptibilities of S. pseudintermedius strains were determined by agar microdilution method according to the CLSI recommendations. All strains were subjected to whole genome sequencing (WGS). Then resistant genes, multilocus sequence typing (MLST), SCCmec types were deduced from WGS data. A total of 75 (12.5%) S. pseudintermedius were isolated from all clinical samples, including 42 (56%) methicillin-resistant S. pseudintermedius (MRSP) and 33 (44%) methicillin-susceptible S. pseudintermedius (MSSP). All of S. pseudintermedius were highly resistant to penicillin, azithromycin, clindamycin, doxycycline, enrofloxacin, ciprofloxacin, chloramphenicol and oxacillin; and were sensitive to linezolid, vancomycin, amikacin and rifampicin. Multi-drug-resistant (MDR) rate of 75 S. pseudintermedius were 90.7% (n=68/75), while all of MRSP isolates (100%, n=42/42) were MDR. Nineteen antimicrobial resistanct genes were detected by WGS. The detection rate of blaZ and aac(6')-aph(2″) were the highest, both were 92% (69/75). A total of 70 sequence types (STs) were detected, and 54 were novel. There were no dominant STs in this study. Among 42 MRSP isolates, 23 (54.7%) belong to staphylococcal cassette chromosome mec (SCCmec) V. This study showed that MDR of MRSP and MSSP were both high, which presents very important issue that MDR-MSSP cannot be ignored; and high genetic diversity among S. pseudintermedius exhibited in China.

Key words: dog, Staphylococcus pseudintermedius, multidrug-resistant, antimicrobial resistance genes, molecular typing

中图分类号:

S852.611

张海霞, 张振彪, 代禾根, 宋昱, 李蕾, 夏兆飞. 中国部分地区犬源伪中间葡萄球菌的流行特征分析[J]. 畜牧兽医学报, 2021, 52(9): 2561-2568.

ZHANG Haixia, ZHANG Zhenbiao, DAI Hegen, SONG Yu, LI Lei, XIA Zhaofei. Analysis of Epidemiological Characteristics of Clinical Staphylococcus pseudintermedius Isolated from Dogs[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(9): 2561-2568.

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