脂肪来源间充质干细胞条件培养基对小型猪腹腔镜肝损伤氧化应激反应的影响

doi:10.11843/j.issn.0366-6964.2021.06.028

摘要/Abstract

摘要： 旨在探究脂肪来源间充质干细胞条件培养基（adipose-derived mesenchymal stem cells-conditioned medium，ADSCs-CM）对小型猪肝损伤氧化应激反应的影响，作者选取24头健康小型猪，随机分为4组，每组6只，分别为模型组（IRI）、DMEM对照组（DMEM）、ADSCs-CM治疗组（CM）和ADSCs治疗组（ADSCs）。4组均通过腹腔镜技术建立小型猪肝缺血再灌注（ischemia reperfusion，IR）合并部分肝切除的肝损伤模型，IRI组移植生理盐水，DMEM组移植浓缩的基础培养基，CM组移植浓缩的脂肪来源间充质干细胞培养基，ADSCs组移植脂肪间充质干细胞。各组分别于术前、术后1、3、7 d采集血液与肝组织样本，使用肝功能检测试剂盒对血清中总胆红素（T-BIL）、乳酸脱氢酶（LDH）、总蛋白（TP）进行检测；使用氧化应激检测试剂盒对肝组织中丙二醛（MDA）、髓内过氧化物酶（MPO）、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GSH-Px）进行检测。结果显示：术后1、3 d：模型组和对照组肝功能严重损伤，发生明显氧化应激反应，CM和ADSCs治疗组显著促进肝功能的恢复，且氧化应激相应指标较模型组和对照组表达明显下降。术后7 d，各组基本恢复到术前水平。结果显示：腹腔镜肝缺血再灌注合并肝部分切除损伤可致小型猪发生氧化应激反应，脂肪来源间充质干细胞及其条件培养基均可改善肝损伤后的氧化应激反应。

关键词: 小型猪, 腹腔镜, 肝缺血再灌注合并部分肝切除损伤, 脂肪间充质干细胞条件培养基, 氧化应激

Abstract: To explore the oxidative stress response of adipose-derived mesenchymal stem cells-conditioned medium (ADSCs-CM) on liver injury in miniature pigs. In this experiment, 24 healthy miniature pigs were selected and randomly divided into four groups, namely model group (IRI), DMEM control group (DMEM), ADSCs-CM treatment group (CM) and ADSCs treatment Groups (ADSCs), six pigs each group. All four groups, laparoscopic techniques were used to establish the liver injury model of miniature pigs with ischemia reperfusion (IR) combined with partial hepatectomy. The pigs in the IRI group were transplanted with normal saline, the pigs in the DMEM group were transplanted with concentrated basal medium, and the pigs in the CM group were transplanted with concentrated adipose-derived stem cell culture medium, the pigs in the ADSCs group were transplanted with adipose stem cells. Blood and liver tissue samples were collected in each group before the operation, 1 day, 3 days and 7 days after the operation. Use liver function test kit to detect total bilirubin (T-BIL), lactate dehydrogenase (LDH), and total protein (TP) in sera; use oxidation reaction kit to detect malondialdehyde (MDA), intramedullary peroxidase (MPO), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase in liver tissue (GSH-Px) for detection. Results were as follows:1 day and 3 days after operation, the model group and the control group had severe liver function damage, with obvious oxidative stress. The CM and ADSCs treatment groups significantly promoted the recovery of liver function, and the expression of oxidative stress related indicators decreased significantly compared with the model group and the control group. After 7 days, each group basically recovered to the preoperative level. The results showed that laparoscopic liver ischemia-reperfusion combined with partial hepatectomy injury can cause oxidative stress in miniature pigs. Adipose-derived mesenchymal stem cells and their condition medium can improve oxidative stress after liver injury.

Key words: miniature pigs, laparoscopy, liver ischemia-reperfusion combined with partial hepatectomy injury, adipose stem cells-conditioned medium, oxidative stress

中图分类号:

S857.12

焦智慧, 张千振, 王月, 刘涛, 刘博洋, 马亚军, 刘笑凝, 朴晨曦, 王洪斌. 脂肪来源间充质干细胞条件培养基对小型猪腹腔镜肝损伤氧化应激反应的影响[J]. 畜牧兽医学报, 2021, 52(6): 1734-1743.

JIAO Zhihui, ZHANG Qianzhen, WANG Yue, LIU Tao, LIU Boyang, MA Yajun, LIU Xiaoning, PIAO Chenxi, WANG Hongbin. Effect of Adipose-derived Stem Cells-conditioned Medium on Oxidative Stress in Miniature Pigs with Laparoscopic Hepatic Injury[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(6): 1734-1743.

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