猫源胎儿三毛滴虫的形态学观察及分子鉴定

doi:10.11843/j.issn.0366-6964.2020.09.031

畜牧兽医学报 ›› 2020, Vol. 51 ›› Issue (9): 2324-2328.doi: 10.11843/j.issn.0366-6964.2020.09.031

• 研究简报 • 上一篇

猫源胎儿三毛滴虫的形态学观察及分子鉴定

柳佳¹, 李永秀¹, 梅汝蕃¹, 王雪敏², 陈丽凤¹, 王秋悦^1*

1. 河北科技师范学院动物科技学院, 河北省预防兽医学重点实验室, 秦皇岛 066000;
2. 河北省张家口市尚义县农业农村局, 张家口 075000

收稿日期:2020-03-23 出版日期:2020-09-25 发布日期:2020-09-25
通讯作者: 王秋悦,主要从事动物寄生虫病学研究,E-mail:13933696970@163.com
作者简介:柳佳(1995-),女,河北三河人,硕士生,主要从事动物寄生虫病学研究;李永秀(1995-),河北衡水人,硕士生,主要从事动物寄生虫病学研究。
基金资助:
河北省青年拔尖人才项目；河北省高校科学技术研究优秀青年基金项目（YQ2014012）

Isolation and Identification of Trichomonas foetus Causing Chronic Diarrhea in Cat

LIU Jia¹, LI Yongxiu¹, MEI Rufan¹, WANG Xuemin², CHEN Lifeng¹, WANG Qiuyue^1*

1. Hebei Normal University of Science and Technology, Key Laboratory of Preventive Veterinary Medicine in Hebei Province, Qinhuangdao 063000, China;
2. Agricultural and Rural Bureau of Shangyi County in Hebei Province, Zhangjiakou 075000, China

Received:2020-03-23 Online:2020-09-25 Published:2020-09-25

摘要/Abstract

摘要： 旨在进一步鉴定从1例长期慢性腹泻猫粪便中新分离到的1株毛滴虫，分别对其进行了分离与体外培养、形态学观察及18S rRNA基因序列相似性分析。形态学观察结果显示，分离株毛滴虫呈梨形或纺锤形，且具有3根前鞭毛和1根后鞭毛，为胎儿三毛滴虫形态结构。18S rRNA基因序列与GenBank中三毛滴虫基因序列比对及系统进化树结果显示，分离的猫源毛滴虫序列与胎儿三毛滴虫MH400076.1处于同一分支，且相似性达到了99%。综合判定，从患有长期慢性腹泻猫的粪便中分离到的毛滴虫为胎儿三毛滴虫。

关键词: 猫, 胎儿三毛滴虫, 分离鉴定

Abstract: To further identify a new strain of Trichomonad isolated from the feces of a cat with chronic diarrhea, isolation and cultivation， morphological observation and gene homology analysis of 18S rRNA of the Trichomonad were accomplished in this study. Morphological observation showed that the Trichomonas was shaped pear or spindle, with 3 anterior flagella and 1 posterior flagella. The results of phylogenetic tree construction showed that Trichomonas isolated in this study is Trichomonas foetus, which is in the same evolutionary branch and is closely related to the sequence MH400076.1 in GenBank with 99% sequence similarity. Based on the above identification results, this Trichomonas isolated from cats with long-term chronic diarrhea is T. foetus.

Key words: cat, Tritrichomonas foetus, isolation, identification

中图分类号:

柳佳, 李永秀, 梅汝蕃, 王雪敏, 陈丽凤, 王秋悦. 猫源胎儿三毛滴虫的形态学观察及分子鉴定[J]. 畜牧兽医学报, 2020, 51(9): 2324-2328.

LIU Jia, LI Yongxiu, MEI Rufan, WANG Xuemin, CHEN Lifeng, WANG Qiuyue. Isolation and Identification of Trichomonas foetus Causing Chronic Diarrhea in Cat[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(9): 2324-2328.

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猫源胎儿三毛滴虫的形态学观察及分子鉴定

Isolation and Identification of Trichomonas foetus Causing Chronic Diarrhea in Cat

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