蛋壳胶护膜性状的遗传特性及其与蛋壳品质性状的关联分析

doi:10.11843/j.issn.0366-6964.2018.04.001

摘要/Abstract

摘要：

旨在通过对某洛岛红蛋鸡纯系的胶护膜品质及其它蛋壳品质指标进行评价，在阐述该性状的重要性及遗传特性的同时分析它与其它蛋壳品质的遗传和表型关联，进而为国产褐壳/粉壳蛋鸡品种的胶护膜性状优化选育、鸡蛋自身抗菌性能的强化提高提供理论支持。本研究测定了北京市华都峪口禽业有限责任公司洛岛红蛋鸡纯系120个家系共计3 283只母鸡的蛋壳胶护膜品质，运用DMU软件估计了其遗传力及其与蛋壳品质性状的遗传相关和表型相关。结果表明，该洛岛红蛋鸡纯系的胶护膜品质为（24.24±9.13）%，应用DMU软件估计其遗传力为0.40，为中等遗传力。蛋壳颜色（L^*、a^*、b^*）、蛋壳强度和蛋壳厚度分别为（61.45±4.91）、（17.79±2.34）、（30.59±2.50），（3.22±0.75）kg·cm^-2和（324.85±42.37）μm，并且都属于中等或中等偏下遗传力性状，范围从0.39到0.17。胶护膜品质与蛋壳颜色（L^*、a^*、b^*）3个指标呈现出不同程度的遗传相关，分别为-0.49、0.43、-0.27；胶护膜品质与蛋壳强度和厚度之间的遗传相关系数分别为0.02和0.13，相关程度较小。蛋壳厚度和蛋壳强度之间的遗传相关较高，为0.72。结果显示，胶护膜性状遗传力较高，对该性状进行直接选择可以获得较快的育种进展。胶护膜品质与蛋壳颜色（L^*、a^*、b^*）具有不同程度的遗传相关，对鸡蛋胶护膜的强化选育可能会同时加深蛋壳颜色。胶护膜品质与蛋壳强度、蛋壳厚度之间的遗传相关较低，说明在选择蛋壳胶护膜性状时不会对蛋壳强度和厚度性状产生明显影响。

Abstract:

In this study, we evaluated cuticle quality and other eggshell quality traits in the Rhode Island Red hens, and we also calculated the genetic and phenotypic correlations between cuticle quality and other eggshells quality traits, which could provide theoretical support for cuticle quality optimization and breeding in the domestic brown/pink egg layer, as well as enhancement of antibacterial properties of egg. We measured cuticle qualities of 3 283 Rhode Island Red hens (120 pedigrees) in Beijing Huadu Yukou Poultry Industry Co. LTD.. Cuticle quality heritability, genetic and phenotypic correlation between cuticle quality and other eggshell quality traits were evaluated by DMU software. The results showed that the value of cuticle quality was (24.24±9.13)%, and its heritability was 0.40 (medium heritability). The values of eggshell color (L^*, a^*, b^*), eggshell strength and eggshell thickness were (61.45±4.91), (17.79±2.34), (30.59±2.50), (3.22±0.75) kg·cm^-2 and (324.85±42.37)μm, respectively. Their heritability were from 0.39 to 0.17, which represented heritability from medium to low. Furthermore, the genetic correlations between cuticle quality and eggshell color (L^*, a^*, b^*) were —0.49, 0.43 and —0.27, respectively. Both eggshell strength and eggshell thickness showed weak correlation with cuticle quality, which were 0.02 and 0.13, respectively. The genetic correlation between eggshell strength and eggshell thickness was 0.72. In conclusion, direct selection of cuticle quality (high heritability) could accelerate genetic progress and improve breeding efficiency. Selection of cuticle quality might deepen eggshell color, since it was related to the L^*, a^* and b^* values. Besides, selection of cuticle quality would not significantly impact eggshell strength and thickness owing to low genetic correlations between them.

中图分类号:

S831.2

李文博, 陈霞, 苑忠央, 迂莹莹, 吴桂琴, 石凤英, 杨宁, 徐桂云, 郑江霞. 蛋壳胶护膜性状的遗传特性及其与蛋壳品质性状的关联分析[J]. 畜牧兽医学报, 2018, 49(4): 659-666.

LI Wen-bo, CHEN Xia, YUAN Zhong-yang, YU Ying-ying, WU Gui-qin, SHI Feng-ying, YANG Ning, XU Gui-yun, ZHENG Jiang-xia. Genetic Characterization of Cuticle and Its Association with Eggshell Quality Traits[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(4): 659-666.

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参考文献

[1] MUÑOZ A, DOMINGUEZ-GASCA N, JIMENEZ-LOPEZ C, et al. Importance of eggshell cuticle composition and maturity for avoiding trans-shell Salmonella contamination in chicken eggs[J]. Food Control, 2015, 55:31-38.
[2] WELLMAN-LABADIE O, PICMAN J, HINCKE M T. Antimicrobial activity of the Anseriform outer eggshell and cuticle[J]. CompBiochem Physiol Part BBiochem Mol Biol, 2008, 149(4):640-649.
[3] HINCKE M, GAUTRON J, RODRIGUEZ-NAVARRO A B, et al. The eggshell:structure and protective function[M]//NYS Y, BAIN M, VAN IMMERSEEL F. Improving the safety and quality of eggs and egg products:Egg chemistry, production and consumption. London:Prentice Hall, Inc., 2011:151-182.
[4] WILSON P W, SUTHER C S, BAIN M M, et al. Understanding avian egg cuticle formation in the oviduct:a study of its origin and deposition[J]. Biol Reprod, 2017, 97(1):39-49.
[5] WHITTOW G C. Sturkie's avian physiology[M]. 5th ed. SanDiego:Academic Press, 2000.
[6] PARSONS A H. Structure of the eggshell[J]. Poult Sci, 1982, 61(10):2013-2021.
[7] MANN K. Proteomic analysis of the chicken egg vitelline membrane[J]. Proteomics, 2008, 8(11):2322-2332.
[8] ROSE-MARTEL M, DU J W, HINCKE M T. Proteomic analysis provides new insight into the chicken eggshell cuticle[J]. J Proteomics, 2012, 75(9):2697-2706.
[9] SAMIULLAH S, ROBERTS J R. The eggshell cuticle of the laying hen[J]. World's Poult Sci J, 2014, 70(4):693-707.
[10] KAGE-NAKADAI E, KOBUNA H, KIMURA M, et al. Two very long chain fatty acid acyl-CoA synthetase genes, acs-20 and acs-22, have roles in the cuticle surface barrier in Caenorhabditis elegans[J]. PLoS One, 2010, 5(1):e8857.
[11] D'ALBA L, TORRES R, WATERHOUSE GIN, et al. What does the eggshell cuticle do? A functional comparison of avian eggshell cuticles[J].Physiol Biochem Zool, 2017, 90(5):588-599.
[12] DOMINGUEZ-GASCA N, MUÑOZ A, RODRIGUEZ-NAVARRO A B. Quality assessment of chicken eggshell cuticle by infrared spectroscopy and staining techniques:a comparative study[J]. Br Poult Sci, 2017, 58(5):517-522.
[13] BIALKA K L, DEMIRCI A, KNABEL S J, et al. Efficacy of electrolyzed oxidizing water for the microbial safety and quality of eggs[J]. Poult Sci, 2004, 83(12):2071-2078.
[14] GOLE V C, ROBERTS J R, SEXTON M, et al. Effect of egg washing and correlation between cuticle and egg penetration by various Salmonella strains[J]. Int J Food Microbiol, 2014, 182-183:18-25.
[15] LOCK J L, BOARD R G. Persistence of contamination of hens' egg albumen in vitro with Salmonella serotypes[J]. Epidemiol Infect, 1992, 108(3):389-396.
[16] BOARD R G, HALLS N A. The cuticle-a barrier to liquid and particle penetration of the shell of the hen's egg[J]. Br Poult Sci, 1973, 14(1):69-97.
[17] LELEU S, MESSENS W, DE REU K, et al. Effect of egg washing on the cuticle quality of brown and white table eggs[J]. J Food Prot, 2011, 74(10):1649-1654.
[18] BAIN M M, MCDADE K, BURCHMORE R, et al. Enhancing the egg's natural defence against bacterial penetration by increasing cuticle deposition[J]. Anim Genet, 2013, 44(6):661-668.
[19] CAVERO D, SCHMUTZ M, ICKEN W, et al.Attractive eggshell color as a breeding goal[J]. Lohmann Inform, 2012, 47(2):15-21.
[20] ALI A. The relationship between eggshell colour and egg quality traits in table eggs[J]. Indian J Anim Res, 2014, 48(3):290-294.
[21] 张龙超. 鸡蛋品质性状遗传参数及相关候选基因研究[D]. 北京:中国农业大学, 2007.
ZHANG L C. Study on genetic parameters for and candidate genes associated with egg quality traits in chickens[D]. Beijing:China Agricultural University, 2007. (in Chinese)
[22] 刘文博. 鸡产蛋和蛋品质性状全基因组关联分析研究[D]. 北京:中国农业大学, 2009.
LIU W B. Genome-wide association studies for egg production and quality traits in egg-type chickens[D]. Beijing:China Agricultural University, 2009. (in Chinese)
[23] INGRAM D R, HATTEN Ⅲ L F, HOMAN K D. A study on the relationship between eggshell color and eggshell quality in commercial broiler breeders.[J]. Int J Poult Sci, 2008, 7(7):700-703.
[24] MERTENS K, VAESEN I, LOFFEL J, et al. The transmission color value:A novel egg quality measure for recording shell color used for monitoring the stress and health status of a brown layer flock[J]. Poult Sci, 2010, 89(3):609-617.
[25] MESSENS W, GRIJSPEERDT K, REU K, et al. Eggshell penetration of various types of hens' eggs by Salmonella enterica serovar Enteritidis[J]. J Food Prot, 2007, 70(3):623-628.
[26] HASIAK R J, VADEHRA D V, BAKER R C. Lipid composition of the eggexteriors of the chicken (Gallus gallus)[J]. Comp Biochem Physiol, 1970, 37(3):429-435.
[27] RODRÍGUEZ-NAVARRO A B, DOMÍNGUEZ-GASCA N, MUÑOZ A, et al. Change in the chicken eggshell cuticle with hen age and egg freshness[J]. Poult Sci, 2013, 92(11):3026-3035.
[28] SAMIULLAH S, ROBERTS J R. The location of protoporphyrin in the eggshell of brown-shelled eggs[J]. Poult Sci, 2013, 92(10):2783-2788.
[29] WALKER A W, HUGHES B O. Eggshell colour is affected by laying cage design[J]. BrPoult Sci,1998, 39(5):696-699.
[30] SAMIULLAH, CHOUSALKAR K K, ROBERTS J R, et al. Effects of egg shell quality and washing on Salmonella Infantis penetration[J]. Int J Food Microbiol, 2013, 165(2):77-83.
[31] LIU Y C, CHEN T H, WU Y C, et al. Effects of egg washing and storage temperature on the quality of eggshell cuticle and eggs[J]. Food Chem, 2016, 211:687-693.
[32] DUNN I C, JOSEPH N T, BAIN M, et al. Polymorphisms in eggshell organic matrix genes are associated with eggshell quality measurements in pedigree Rhode Island Red hens[J]. Anim Genet, 2009, 40(1):110-114.